Abstract: he objective of this study was to investigate the effect of bovine flow cytometrically-sorted sperm after two treatments. Bovine oocytes were randomly divided into two groups and matured and inseminated in vitro with washing or Percoll treatment. Sperm survival rate and density were recorded following treatments. The results showed that: there was no significant difference in sperm survival and blastocyst rates between the two treatment groups, but washing treatment resulted with higher sperm density compared to percoll treatment; sperm density with washing treatment was significantly higher than that with Percoll treatment (P<0.01). The present studydemonstrated that the efficiency of utilization of bovine flow cytometricallysorted sperm can be improved with washing treatment as it can obtain higher sperm denesity.

Key words: sorted sperm; in vitro fertilization(IVF); washing treatment; Percoll treatment