2拷贝SLT-ⅡvB在猪霍乱沙门氏菌C500crp-/asd-缺失株中的表达及小鼠免疫试验

Abstract

Abstract: In order to develop novel piglet’s Paratyphoid-ED bivalent vaccine, SLT-ⅡvB gene without signal peptide was amplified from pMDSLT-Ⅱ and subcloned into pYA3493. pYA2B was electroporated into C500crp-/asd- mutant. SDS-PAGE and Western blotting were done. Mice were i.p. immunized with C500crp-/asd-(pYA2B) and C500crp-/asd-(pYA3493) and i.p. challenged with 2 LD50 ED3 and 10 LD50 C78-1. 2 SLT-ⅡvB was expressed in C500crp-/asd- mutant. The C500crp-/asd-(pYA2B) immunized group was 60% protected mice against ED3 challenging and control groups couldn’t protect mice. All immunized groups induced 100% protected mice against C78-1 challenging. These results provided base for developing piglet’s Paratyphoid-ED bivalent vaccine and further improving of immunity effect.

Key words: S. choleraesuis C500crp-/asd- mutant; balanced lethal host-vector system; 2 copies SLT-ⅡvB; gene expression; immune test

CLC Number:

S852.61

XU Yin-di;WANG Zhi-fang;ZHU Wen-hao;GUO Cheng-liu. Expression of 2 Copies SLT-ⅡvB Gene in C500crp-/asd- Mutant of Salmonella choleraesuis and Immune Test on Mice[J]. , 2010, 37(7): 70-73.

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Expression of 2 Copies SLT-ⅡvB Gene in C500crp-/asd- Mutant of Salmonella choleraesuis and Immune Test on Mice

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