槲皮素对蒙古羊卵泡发育、卵泡液微环境及卵巢类固醇生成相关基因表达的影响

doi:10.16431/j.cnki.1671-7236.2025.03.017

Abstract

Abstract: 【Objective】 The aim of this study was to explore the effects of quercetin on follicular development,immune and antioxidant function,follicular fluid reproductive hormone secretion and ovarian steroidogenesis related genes in ewes.【Method】 In this study,thirty Mongolian ewes with similar body weight were randomly divided into two groups:Control group and quercetin group.The control group of ewes were fed with basic diet,and each ewe in quercetin group was fed a basal diet supplemented with quercetin 7 g/d.Each group had three replicates,and five sheep in each replicate were raised in a separate pen.Blood samples were collected on the 13th day of the second estrus stage for the detection of antioxidant indexes and reproductive hormone indexes,and ovarian tissues were collected for the detection of steroidogenesis related gene expression.【Result】 ①Compared with control group,the number of large and middle follicles in quercetin group increased significantly(P＜0.05),the levels of interleukin-6,tumor necrosis factor,immunoglobulin G,and immunoglobulin A in serum of ewes in quercetin group were extremely significantly or significantly reduced (P＜0.01 or P＜0.05),while the total antioxidant capacity,superoxide dismutase,and glutathione peroxidase activities in serum were extremely significantly or significantly increased (P＜0.01 or P＜0.05).②Compared with control group,the levels of estrogen,progesterone,follicle stimulating hormone and gonadotropin-releasing hormone in the large follicles of ewes in quercetin group were extremely significantly or significantly higher than those in control group(P＜0.01 or P＜0.05).The levels of estrogen and follicle stimulating hormone in medium follicles were extremely significantly increased(P＜0.01).The levels of luteinizing hormone were significantly or extremely significantly reduced in the follicles at different grades (P＜0.05 or P<0.01).③Compared with control group,the mRNA expression of steroidogenic acute regulatory protein (STAR) and cytochrome P450 family 19 subfamily A member 1 (CYP19A1) in ewe ovaries were extremely significantly upregulated by adding quercetin to the feed (P＜0.01).【Conclusion】 Adding 7 g/d quercetin to the feed could effectively improve the anti-inflammatory and antioxidant functions of ewes,and regulate the secretion of reproductive hormones in follicular fluid and the expression of genes related to ovarian steroid production,thereby affecting the development of ewe follicles.

Key words: quercetin; follicular development; follicular fluid microenvironment; steroid hormones; gene expression

CLC Number:

S814.1

WANG Yong, NA Risu, LIANG Jianyong, LIU Yang, LI Jiuyue, GAO Yuan, TIAN Feng, XUE Shuyuan. Effects of Quercetin on Follicular Development,Follicular Fluid Microenvironment and Ovarian Steroidogenesis Related Gene Expression in Mongolian Sheep[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(3): 1150-1158.

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Effects of Quercetin on Follicular Development,Follicular Fluid Microenvironment and Ovarian Steroidogenesis Related Gene Expression in Mongolian Sheep

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