CAPN3基因在草原红牛不同组织中的表达差异

›› 2010, Vol. 37 ›› Issue (10): 105-108.

• 生物技术 • Previous Articles Next Articles

Expression and Comparative Study of Calpain3 (CAPN3) Gene in Different Tissues of Chinese Red Steppe

LIU Bo-yang1, QIN Li-hong1, LIAN Chuan-jiang1, TIAN Jing1, ZHANG Jin-yu1, JIANG Hao2, ZHAO Zhi-hui1, YANG Run-jun1

(1.College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China;2.Centre of Experimental Animal, Jilin University, Changchun 130062, China)

Received:1900-01-01 Revised:1900-01-01 Online:2010-10-20 Published:2010-10-20
Contact: YANG Run-jun

Abstract

Abstract: The present study was designed to detect the differences of the calpain 3 (CAPN3) gene expression level in varieties tissues of Chinese Red Steppe by real-time RT-PCR. The results showed that CAPN3 mRNA was expressed in all of the eight tissues: heart, liver, spleen, lung, kidney, intestine, stomach and skeletal muscle; we also found that CAPN3 gene expression level was higher in spleen than in other tissues of Chinese Red Steppe (P<0.05). The research provides the basis for the future studies of the biological function and the molecular mechanisms of CAPN3 gene.

Key words: Chinese Red Steppe;  CAPN3; real-time PCR; expression level

CLC Number:

LIU Bo-yang;QIN Li-hong;LIAN Chuan-jiang;TIAN Jing;ZHANG Jin-yu;JIANG Hao;ZHAO Zhi-hui;YANG Run-jun. Expression and Comparative Study of Calpain3 (CAPN3) Gene in Different Tissues of Chinese Red Steppe[J]. , 2010, 37(10): 105-108.

[1]	MA Baoyi, SHENG Chenyan, LIU Hongying, MA Qingxia, WANG Tingting, LI Jianming, SHI Kun, DU Rui, LENG Xue. Establishment of a Duplex TaqMan Real-time PCR Method for Detection of Bovine Viral Diarrhea Virus Types 1 and 2 [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2326-2335.
[2]	XUE Jiajia, QIN Lihong, CAO Yang, GAO Yi, LIU Lixiang, LI Xu, XIAO Cheng, WANG Jing, ZHANG Guoliang. Cloning of Ephrin A5 Gene and Its Expressing in Different Tissues in Red Steppe [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(6): 1967-1975.
[3]	LI Jianmei, JI Zhengjian, LIU Mei, SHEN Xinyue, CHENG Xu, YU Yan, DAI Yabin. Expression Changes of Immune-related Genes in Tibetan Chickens and Recessive White Chickens Following Eimeria tenella Infection [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(4): 1440-1448.
[4]	JIANG Hui, LIU Pan, LI Yuanyuan, LIU Kuiguo, HUANG Xixia, LIU Jiangwei, XU Lei, DU Jianwen, SANG Zhagen, YANG Xuemei, ZHAO Fanfan, WANG Dan. Expression and Bioinformatics Analysis of Mastitis Resistance Related Genes in Xinjiang Brown Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(1): 90-97.
[5]	XU Min, XU Houqiang, CHEN Wei, YANG Yang. Study on Expression of the Relate Genes Involved in Intramuscular Preadipocyte Differentiation in Congjiang Xiang Pig [J]. , 2018, 45(9): 2492-2499.
[6]	ZHAO Xueli, LIU Yi, BAN Fuguo, YAN Ruoqian, WANG Huajun, WANG Shujuan, MA Zhenyuan, WANG Dongfang. Establishment and Application of A Double TaqMan MGB Real-time PCR Assay for Detection of Canine Distemper Virus and Canine Parvovirus [J]. , 2018, 45(8): 2086-2094.
[7]	LAN Desong, GU Guibo, HOU Zhengzhong. Development of a TaqMan Real-time PCR for Differentiation of Wild-type Strain from gE-deleted Vaccine Strain of Pseudorabies Virus [J]. , 2018, 45(7): 1804-1812.
[8]	GAO Xintao, JIA Hong, HOU Shaohua, GUO Xiaoyu, YUAN Weifeng, JIANG Yitong, ZHU Hongfei, XIN Ting. Research on the Potential of IL-17 as a Biomarker of Bovine Tuberculosis and Establishment of Its Real-time PCR Assay [J]. , 2018, 45(6): 1447-1453.
[9]	RUAN Yiqi, LI Dapeng, RAN Xueqin, MAO Ning, YI Fanli, ZHANG Fuping, WANG Jiafu. The Methylation in the Exon 1 of RARRES1 Gene and its Association with Gene Differential Expression of Xiang Pig Ovary [J]. , 2018, 45(6): 1471-1478.
[10]	MAO Ning, WANG Jiafu, ZHANG Fuping, TANG Liangting, RUAN Yiqi, YI Fanli, RAN Xueqin. Study on Differential Expression of StAR and CYP11A1 Genes in Xiang Pig Ovary [J]. , 2018, 45(5): 1137-1144.
[11]	WAN Chunhe, LIU Rongchang, CHENG Longfei, SHI Shaohua, FU Guanghua, CHEN Hongmei, FU Qiuling, HUANG Yu. Establishment of a TaqMan-based Real-time PCR Method of Duck Adenovirus A [J]. , 2018, 45(5): 1341-1348.
[12]	ZHANG Mengyao, YANG Feng, LIU Kaidong, CHENG Ming, WANG Guoyi, LIU Jifeng, LIU Nan, HE Jianning. Study on Construction of DKK1 Gene Recombinant Plasmid in Aohan Fine Wool Sheep and Its Expression in Fibroblasts [J]. , 2018, 45(1): 131-139.
[13]	BU Xiao-yan, LI Hui, SHI Xiao-li, SHI Xin-cai, ZHAO Zhong-hai, CHEN Lin. Study on Different Expression of HO-1 Gene of 70-weeks-old Changshun Blue-shelled Chicken in Different Tissues [J]. , 2017, 44(6): 1762-1768.
[14]	LI Yuan-ji, YANG Ming-hua, LIU Fang, ZHU Fang-fang, HE Shao-yu, KUANG Hai-ou, LI Ya-hui. cDNA Cloning, Tissue Expression Profiles and Bioinformatical Analysis of Tssk6 Gene in Tree Shrews [J]. , 2017, 44(3): 619-627.
[15]	CAI Wen-yi, CHEN Tong, SHI Xian, CHEN Wei-ming, HU Jia-jia, XIONG Xian-rong, LAN Dao-liang, LI Jian. Cloning of PLIN2 Gene of Yak and Its Expression in Ovary during Different Estrus Periods [J]. , 2017, 44(3): 782-789.

Expression and Comparative Study of Calpain3 (CAPN3) Gene in Different Tissues of Chinese Red Steppe

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments