4个家兔品种MHC-DQA基因外显子2 Mbo Ⅱ酶切多态性分析

Abstract

Abstract: Genetic polymorphism on exon 2 of MHC-DQA gene was investigated in 4 rabbits breeds by PCR-RFLP method with Mbo Ⅱ enzyme, and cluster analysis was also performed. The results showed that there was a polymorphism at position 103 bp of exon 2 of MHC-DQA. The χ² tests showed that Mbo Ⅱ restriction enzyme sites were not in Hardy-Weinberg equilibrium among ZIKA large New Zealand white rabbits and ZIKA giant white rabbits(P＜0.05), while in Hardy-Weinberg equilibrium among California rabbit and Qixin rabbit(P＞0.05), cluster analysis showed that ZIKA large New Zealand white rabbits had the closest genetic relationship with ZIKA giant white rabbits. The results obtained in this study will provide a molecular biological basis for further analysis of relationship of MHC polymorphism and disease resistance in rabbit.

Key words: rabbit; MHC-DQA; genetic polymorphism

CLC Number:

S829.1

KUANG Liang-de;XIE Xiao-hong;HUANG Deng-ping;YI Jun;LEI Min;REN Yong-jun;GUO Zhi-qiang;LI Cong-yan;ZHENG Jie. Polymorphism Analysis on Exon 2 of MHC-DQA Gene in 4 Rabbit Breeds[J]. , 2011, 38(4): 137-139.

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Polymorphism Analysis on Exon 2 of MHC-DQA Gene in 4 Rabbit Breeds

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