不同生长速度撒坝猪背最长肌lncRNA筛选与功能预测

doi:10.16431/j.cnki.1671-7236.2025.06.004

Abstract

Abstract: 【Objective】 This study was aimed to identify the long non-coding RNA (lncRNA) in longissimus dorsi muscle tissue of Saba pigs with fast and slow growth rates,and explore its important roles in pig growth and development.【Method】 Six Saba pigs (three males and three females) with the largest and smallest body weight at 322 days were selected and divided into fast-growth group (FG) and slow-growth group (SG),and the longissimus dorsi muscle samples were collected for RNA-Seq,respectively.After quality control,alignment and splicing of the sequencing data,the DESeq2 software package was used to identify the differentially expressed lncRNA in FG and SG.Target genes were further predicted and analyzed for GO function and KEGG pathway enrichment.Six differentially expressed lncRNAs were randomly selected for Real-time quantitative PCR verification.【Result】 A total of 165 differentially expressed lncRNAs were identified,of which 71 were up-regulated and 94 were down-regulated,and 65 target genes were predicted.The results of GO function and KEGG pathway enrichment analysis showed that the target genes were significantly enriched in functional terms such as lipid metabolism process,canonical Wnt signaling pathway,as well as in pathways including Wnt signaling pathway,arginine and proline metabolism,histidine metabolism,etc.Real-time quantitative PCR results showed that the expression trends of six differentially expressed lncRNAs (ENSSSCT00000085548,MSTRG.8918.1,MSTRG.20943.30,MSTRG.7048.7,MSTRG.11735.9 and MSTRG.20943.40) were consistent with RNA-Seq results,there were significant difference in longissimus dorsi muscle between FG and SG in Saba pigs (P＜0.05).【Conclusion】 lncRNAs identified in this study were related to the process of muscle growth and development,and these lncRNAs could affect growth traits such as average daily weight gain by regulating the expression of genes such as WNT10B,SFRP4,LEP,MAOB,ARG1,PRICKLE1 and DUSP1,which might lead to the differences in growth rate within the population of Saba pigs.The results provided a theoretical basis for further revealing the molecular mechanism of growth and development and the genetic improvement of growth traits such as average daily gain in pigs.

Key words: Saba pigs; longissimus dorsi muscle; RNA-Seq; lncRNA; growth and development

CLC Number:

S813.3
Q78

REN Hao, ZHU Yixuan, CHAO Tingting, WANG Xiaoyi, LU Shaoxiong, YANG Yongli, CHEN Qiang, LI Mingli. Identification and Functional Prediction of lncRNA in Longissimus Dorsi Muscle of Saba Pigs with Different Growth Rates[J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(6): 2494-2505.

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Identification and Functional Prediction of lncRNA in Longissimus Dorsi Muscle of Saba Pigs with Different Growth Rates

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