PLPP3基因多态性对北京黑猪背部脂肪厚度和脂肪酸含量的影响

doi:10.16431/j.cnki.1671-7236.2025.01.025

Abstract

Abstract: 【Objective】 The purpose of this study was to explore the polymorphisms of phospholipid phosphatase 3 (PLPP3) gene and its correlation with back fat thickness and fatty acid content of Beijing Black pigs,in order to provide reference for genetic improvement of back fat thickness and fatty acid content of Beijing Black pigs. 【Method】 In this study,the ear tissue samples of 239 Beijing Black pigs were collected to obtain genomic resequencing data.The thickness of back fat in different parts of 239 Beijing Black pigs (the thickest of the shoulder,junction of the 6-7 thoracic vertebrae,thoracolumbar junction,the joint of the lumbar sacral) and the contents of 5 fatty acids (palmitic acid,stearic acid,oleic acid,linoleic acid,α-linolenic acid) in the back fat of junction of the 6-7 thoracic vertebrae of the left carcass were determined.The sequencing results were utilized to study the population genetic characteristics and haplotype linkage of single nucleotide polymorphism (SNP) sites in the PLPP3 gene,and conduct association analysis between SNPs and back fat thickness and fatty acid content in various parts.The transcription factor of SNP in promoter region were predicted,and the differential gene expression in back fat of individuals with different genotypes of PLPP3 functional SNPs were detected by Real-time quantitative PCR. 【Result】 A total of 8 SNPs of PLPP3 gene were detected in the Beijing Black pig population,of which 5 were moderate polymorphisms (0.25＜PIC＜0.5) and 3 were low polymorphisms (PIC＜0.25),g.155894136 C＞T was in Hardy-Weinberg equilibrium (P＞0.05).Haplotype linkage analysis showed that there was a strong linkage between g.155800228 G＞A and g.155800259 A＞C (D’=91%).There was a certain linkage between g.155891807 G＞A and g.155891715 A＞G(D’＞80%).The association analysis between gene polymorphism and backfat thickness showed that there were significant differences in back fat thickness among individuals with different genotypes,including g.155800222 C＞A,g.155891715 A＞G,g.155891807 G＞A,g.155894136 C＞T (P＜0.05).The association analysis between gene polymorphism and fatty acid content showed that there were significant differences in the content of palmitic acid and the ratio of linoleic acid/α-linolenic acid in back fat among individuals with different genotypes,including g.155800222 C＞A,g.155800228 G＞A (P＜0.05).TFs prediction of SNPs in promoter region found that the mutation of g.155800228 G＞A caused the binding transcription factor to change from SPI1 to MYB.Further analysis of PLPP3 gene expression differences among different genotypes at g.155800228 G＞A showed that PLPP3 gene mRNA level in AA individuals was significantly higher than that in GG individuals (P＜0.05). 【Conclusion】 In summary,the different genotypes of PLPP3 gene were significantly differences with the thickness of back fat and the content of some fatty acids in different parts of Beijing Black pigs.It could be used as a potential molecular marker of back fat thickness and fatty acids content of Beijing Black pigs,and provided theoretical support for breeding of Beijing Black pigs.

Key words: Beijing Black pigs; PLPP3 gene; back fat thickness; fatty acids

CLC Number:

S813.1

ZHU Xueli, MA Jianing, XU Ke, SUN Chao, LIU Xin, PU Lei. Association Analysis of PLPP3 Gene Polymorphisms with Back Fat Thickness and Fatty Acids Content in Beijing Black Pigs[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(1): 278-288.

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Association Analysis of PLPP3 Gene Polymorphisms with Back Fat Thickness and Fatty Acids Content in Beijing Black Pigs

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