性别对杜陆猪肉品质及风味影响的代谢组学分析

doi:10.16431/j.cnki.1671-7236.2021.10.009

Abstract

Abstract: The purpose of the experiment was to comprehend the metabolic differences of sex and the sex effects of meat quality and flavor caused by these metabolic differences of different tissues in Dulu pig, and offer basal metabolic information for meat quality and flavor research in pigs. Sixteen (half male and half female) 8-month-old healthy Dulu pigs with similar body weight were selected, and Non-targeted metabolites detection was conducted in serum, liver, longissimus dorsi muscle by gas chromatography/mass spectrometry (GC/MS). The different metabolites selection and metabolism pathway enrich analysis were conducted to analysis the sex effects of meat quality and flavor by metabonomics. The results showed that sexes had some influence on metabolic profiling of serum, liver, and longissimus dorsi muscle. The numbers of different metabolites caused by sex were bigger in serum and liver, and it was the least in longissimus dorsi muscle. The contents of most lipid differential metabolites in serum of male were higher than female (P<0.05), while it was the opposite in the liver (P<0.05). The contents of most carbohydrate differential metabolites in both serum and liver of male were higher than female (P<0.05). The levels of 2-monoolein, behenic acid, cholesterone and dihydrocholesterol in serum of male were more than 1.5 times as much as female. The levels of beta-gentiobiose, L-kynurenine and L-tyrosine in liver of male were 29, 11 and 3 times as much as female, respectively. Lipoic acid of male was significantly lower than female (P<0.05), which was 50% of female. The pathways of hormone, lipid, carbohydrate and animo acid were significantly different between the males and females (P<0.05). In conclusion, the sex effects were different in serum, liver and longissimus dorsi muscle. There were sex effects on hormone, lipid, carbohydrate and animo acid metabolism in serum and liver, however, these different sex effects were not too strong to reflect meat quality and flavor. The higher levels of lipid and carbohydrate metabolites made the meat quality and flavors of male a little better than female.

Key words: sex; Dulu pig; metabonomics; meat quality; meat flavor

CLC Number:

S828

ZHANG Xingyan, LAN Haien, XIE Bingkun, PAN Tianbiao, CHEN Baojian, GUAN Zhihui, CHEN Zhonghua. Metabonomics Analysis of Sex Effects on Meat Quality and Flavor in Dulu Pig[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(10): 3585-3594.

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Metabonomics Analysis of Sex Effects on Meat Quality and Flavor in Dulu Pig

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