关岭牛解偶联蛋白3基因5’侧翼区克隆和生物信息学分析

Abstract

Abstract: The experiment was conducted to clone 5'-flanking region of bovine UCP3 gene to study regulatory mechanism of the specific transcriptional regulation region. The 5'-flanking region of bovine UCP3 gene was amplified with PCR, and then the 1080 bp 5'-flanking promoter sequence was obtained by product purification, ligation, transformation, and sequence comparison. The obtained cloning vector of 5'-flanking region of bovine UCP3 gene was analyzed by the promoter software in this study. The results indicated that there were 6 transcription start sites and 33 potential transcription factor binding promoter region of the gene, the highly conserved region was in -196～+100 bp of the upstream of transcription start sites, which might be conclude that the -200～+1 bp of the upstream of transcription start sites was the core. Comparing bovine with Panthera tigris, Felis catus, Bubalus bubalis, Pantholops hodgsonii, Capra hircus, Ovis aries,the homology of 5'-flanking promoter sequence were 82%, 82%, 98%, 95%, 96% and 98%, respectively. The 1080 bp 5'-flanking region of the bovine UCP3 gene was cloned successfully and the core promoter region was preliminary predicted in the gene.

Key words: Guanling cattle; UCP3 gene; promoter; transcription factors

CLC Number:

Q785

CHEN Wei, XU Hou-qiang, LIU Zhong-wei, CHEN Xiang, ZHANG Wen, HUAN Cong-cong. Cloning and Sequence Analysis of UCP3 Gene 5’-Flanking Region of Guanling Cattle[J]. , 2014, 41(9): 6-10.

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Cloning and Sequence Analysis of UCP3 Gene 5’-Flanking Region of Guanling Cattle

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