贵州地方山羊BMPR-ⅠB基因启动子区多态性及生物信息学分析

doi:10.16431/j.cnki.1671-7236.2020.04.004

Abstract

Abstract: In order to explore the polymorphism of bone morphogenetic protein receptor ⅠB (BMPR-ⅠB) gene promoter region and its correlation with reproductive traits,three Guizhou local goats,including Qianbei Ma goats,Guizhou Black goats and Guizhou White goats were selected as experimental materials.SNPs were screened by constructing a mixed DNA pool combined with PCR product direct sequencing technology,and various bioinformatics software were used to predict the effects of SNPs on the core promoter region,CpG island and transcription factor binding sites.The results showed that there were 4 SNPs in the promoter region of BMPR-ⅠB gene,which were g.29893005 T > C,g.29893016 C > T,g.29893729 C > G and g.29894370 C > T.The bioinformatics software predicted the core promoter region and CpG island of BMPR-ⅠB gene,and the SNPs site caused the transcription factor binding site to change.The g.29893005 T > C and g.29893016 C > T mutations caused the original transcription factors site binding Sp1 to disappear,g.29893729 C > G mutation caused the original transcription factor binding site AP-1 to disappear,resulting in a new transcription factor binding site USF.g.29894370 C > T mutation produced a new transcription factor binding site C/EBPalp changed the original transcription factor binding site Sp1 to C/EBPalp and Sp1.It was speculated that SNPs might have important effects on regulatory promoter functional elements.

Key words: Guizhou local goats; BMPR-ⅠB gene; promoter; bioinformatics

CLC Number:

S813.3

AI Jinxin, LONG Anju, LUO Weixing, CAI Huifen. Polymorphism and Bioinformatics Analysis of BMPR-ⅠB Gene Promoter Region in Guizhou Local Goats[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(4): 1000-1008.

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Polymorphism and Bioinformatics Analysis of BMPR-ⅠB Gene Promoter Region in Guizhou Local Goats

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