丁鲑鱼鳗弧菌16S rDNA基因及耐药性分析

Abstract

Abstract: Strain SK-1 was isolated from naturally infected Tincaeus. Its morphological, physiological and biochemical experiments, 16S rDNA sequence were performed, and its antibiotic sensitivity was analyzed by Kriby-Bauer disc diffusion method. The results showed that strain SK-1 was gram negative bacteria,with short rod shape and oxidase-positive.Its physiological and biochemical characteristics were consistent with the physiological and biochemical characteristics of Vibrio anguillarum. The length of this 16S rDNA sequence was 1447 bp, which showed 99% homology with Vibrio anguillarum, GenBank database number was KF978124. Phylogenetic analysis indicated it grouped together with Vibrio anguillarum. So it was confirmed that strain SK-1 was Vibrio anguillarum. The antibiotic sensitivity presented that strain SK-1 was highly sensitive to 13 antibiotics such as doxycycline,tetracycline,minocycline, and middle sensitive to 9 antibiotics such as streptomycin, ceftriaxone, trimethoprim, and resistant to bacitracin and lincomycin. These results will be beneficial to the identification of Vibrio anguillarum and prevention and control of bacterial disease in Tincaeus.

Key words: Tincaeus; Vibrio anguillarum; 16S rDNA; drug resistance

CLC Number:

S948

LI Xue-feng, WANG Li, WANG Hai-juan, ZHAO Jing. 16S rDNA Gene and Drug Resistance Analysis of Vibrio anguillarum Isolated from Tincaeus[J]. , 2014, 41(9): 235-239.

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16S rDNA Gene and Drug Resistance Analysis of Vibrio anguillarum Isolated from Tincaeus

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