肠上皮紧密连接蛋白mRNA的表达与 仔猪断奶腹泻关系的研究

Abstract

Abstract: The experiment was carried out to compare diarrhea status between Min pig and Landrace pig in a week after weaning, detect two varieties piglets jejunum, ileum and colon tight proteins Zonula occludens-1(ZO-1)and Occludin mRNA expression before and after weaning. The intention was to discuss the relationship between tight proteins expression and piglets weaning diarrhea. The results showed that Min piglets diarrhea rate, diarrhea frequency were very significantly lower than Landrace pig within one week after weaning (P<0.01), diarrhea days was significantly lower than Landrace pig within one week after weaning (P<0.05);Min piglets not weaning group ileum and colon ZO-1 and Occludin mRNA expression was very significantly higher than Landrace pig not weaning group (P<0.01);Min piglets weaning diarrhea group ileum and colon ZO-1 and Occludin mRNA expression was significantly higher than weaning health group (P<0.05). The results indicated that Min piglets intestinal tight junction proteins mRNA high expression before weaning might be had relation with guarantee piglets intestinal health and decrease occurrence diarrhea;Min pig fast recover intestinal mucosa function and rehabilitation by increased tight junction proteins mRNA expression after weaning. So tight junction proteins mRNA high expression before and after weaning had an important significance for piglets to resist weaning diarrhea.

Key words: ZO-1; Occludin; Min pig; Landrace pig; weaning diarrhea

CLC Number:

S856.4

ZHANG Ying-chao, WANG Xi-biao, CUI Shi-quan, DI Sheng-wei, GUAN Sen. Study on Relationship between Intestinal Epithelium Tight Junction Proteins mRNA Expression and Piglets Weaning Diarrhea[J]. , 2014, 41(9): 221-224.

References

1 王丽姣,周国华. 肠黏膜机械屏障的研究进展[J]. 医学综述, 2011, 17(24): 3702～3704.

2 叶强,欧希龙. 应激致肠道屏障功能损伤的机制[J].现代医学,2011,39(5):619～621.

3 刘光富,初晓娜,汪以真.仔猪早期断奶对肠道屏障损伤机制研究进展[J].养猪, 2004(3): 9～11.

4 刘海萍,胡彩虹,徐勇. 早期断奶对仔猪肠通透性和肠上皮紧密连接蛋白Occludin mRNA表达的影响[J]. 动物营养学报, 2008, 20(4): 442～446.

5 许玲芬,董亚路,孙梅,等. PAF对肠上皮细胞紧密连接的影响及ITF的保护作用[J].世界华人消化杂志, 2008, 16(4): 372～378.

6 李华,郑秀.紧密连接蛋白与妇科肿瘤的相关研究[J].医学综述,2007, 13(10): 655～657.

7 吴银宝,汪植三. 早期断奶仔猪腹泻的研究概述[J]. 家畜生态, 1999, 20(3): 45～48.

8 吴善斌,王学清,郭鸿飞,等. Occludin蛋白在重症急性胰腺炎小鼠肠上皮细胞的表达[J]. 中国医科大学学报, 2011, 40(3): 217～219.

9 胡翔,贺德. 肠黏膜生物屏障研究进展[J]. 中国医学工程, 2010, 18(2): 173～176.

10 胡彩虹,钱仲仓,刘海萍,等.高锌对早期断奶仔猪肠黏膜屏障和肠上皮细胞紧密连接蛋白表达的影响[J].畜牧兽医学报,2009,40(11):1638～1644.

11 姜伟炜,张文远. 紧密连接蛋白与炎症性肠病[J]. 国际消化病杂志, 2010, 30(2): 99～100.

12 夏天. 紧密连接蛋白Claudin-1在大肠杆菌性腹泻仔猪胃肠组织中的表达及意义[D].武汉:华中农业大学,2010.

13 秦环龙,高志光. 肠上皮细胞紧密连接在肠屏障中的作用进展[J]. 世界华人消化杂志, 2005, 13(4): 443～447.

14 蒋义,贾刚,惠明弟,等. 胰高血糖素样肽-2对断奶仔猪肠上皮紧密连接蛋白相关基因表达的影响[J]. 动物营养学报, 2012, 24(9): 1785～1792.

15 雷军,夏祥国.紧密连接蛋白Claudin的研究进展[J].泸州医学院学报, 2009, 32(6):725～727.

16 Barrett K E. New ways of thinking about (and teaching about) intestinal epithelial function[J]. Adv Physiol Educ, 2008, 32(1): 25～34.

17 Hicks T A,McGlone J J,Whisnant C S. Behavioral, endocrine, immune, and performance measures for pigs exposed to acute stress[J]. J Anim Sci, 1998, 76(2): 474～483.

18 Julian A, Guttman A, Finlay B B. Tight junctions as targets of infectious agents[J]. Biochim Biophys Acta, 2009, 1788(4):832～841.

19 Tsukita S, Furuse M, Itoh M. Multifunctional strands in tight junctions[J]. Nat Rev Mol Cell Biol, 2001, 2(4): 285～293.

20 Turner J R. Molecular basis of epithelial barrier regulation:From basic mechanisms to clinical application[J]. Am J Pathol, 2006, 169(9): 1901～1909.

21 Ukena S N, Singh A, Dringenberg R, et al. Probiotic Escherichia coli Nissle 1917 inhibits leaky gut by enhancing mucosal integrity[J]. PLoS One, 2007, 2(12): e1308.

22 Zhang B K,Guo Y M. Supplemental zinc reduced intestinal permeability by enhancing occluding and zonula occludens protein-1 (ZO-1) expression in weaning piglets[J]. British Journal of Nutrition, 2009, 102(5): 687～693.

[1]	GAO Beiyao, LIU Yanguang, JIA Qi, LIU Jianqiang, LUO Xinhui, ZHANG Lichun. Study on Polymorphism and Differential Expression of PBD-124 Gene in Different Breeds of Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2208-2215.
[2]	SUN Changchao, GAO Xiang, LI Feng, LIANG Tong, NING Hangyi, WANG Zhongyu, SHAN Anshan, SHI Baoming. Effects of Short-term Administration of Bacillus velezensis M2 Isolated from Min Pigs on the Growth,Immunity and Intestinal Microflora of SD Rats [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(4): 1264-1274.
[3]	LIU Yanguang, LUO Xinhui, LIU Jianqiang, JIA Qi, XIAO Cheng, CAO Yang, JIN Haiguo, ZHANG Lichun. Analysis of Polymorphism and Expression Profile of PBD-110 Gene in Pigs with Different Genetic Background [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(3): 975-981.
[4]	FU Chunni, LI Yuanhui, LI Pengcheng, LI Liuan, QIN Shunyi. Effects of Selenium-oligochitosan Against Zearalenone on the Expression of Tight Junction Protein in IPEC-J2 Cells [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(11): 4311-4318.
[5]	ZHANG Dongjie, WANG Liang, LIU Yang, LIU Di. Screening and Analysis of Transcription Factors Regulating Tβ4 Gene in Min Pig [J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(9): 2535-2542.
[6]	SONG Chunjie, JI Mei, XU Jia, HUO Bin, CHI Yongkuan, SHEN Xiaoyun. Study on the Therapeutic Effect of Modified Yujin Powder on Transimissible Gastroenteritis in Landrace Pigs [J]. , 2019, 46(2): 600-607.
[7]	QU Qiuhong, XIE Hongyue, XIA Qin, PAN Peng, JIANG Qinyang, HUANG Yanna. Cloning and Bioinformatics Analysis of Occludin Gene in Luchuan Pig [J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(11): 3144-3153.
[8]	HU Wei, LI Mingli, ZHANG Yanlin, YAN Dawei, LI Zhongchang, DONG Xinxing, JI Chunlv, LAN Guoxiang, PAN Yuchun, LU Shaoxiong. Polymorphism of prepro-orexin Gene and Its Association Analysis with Feeding and Growth Traits in Landrace Pigs [J]. , 2018, 45(3): 712-720.
[9]	ZHANG Li-chun, LI Zhao-hua, LI Meng-shu, SUN Fu-liang, CAO Yang, PIAO Qing-lin, JIN Hai-guo, ZHANG Shu-min. The Polymorphism Analysis of Mx1 gene in Pig Populations from Different Genetic Background [J]. , 2017, 44(4): 1046-1053.
[10]	HU Hui-yan, JIA Qing, HOU Sheng-kui, LIU Jin, ZHAO Si-si, LI Xiao-min, ZHANG Wei-feng, LI Shu-yang, WANG Gui-jiang. The Association Analysis Between 4 Major Genes and Reproductive Traits in British Landrace Pigs [J]. , 2017, 44(4): 1087-1094.
[11]	MA Hong, LIU Yu, WANG Liang, WANG Wen-tao, WU Sai-hui, HE Xin-miao, LIU Di. Screening of the Reference Genes for the Study of Peripheral Blood Mononuclear Cells by Quantitative Real-time PCR in Min Pigs [J]. , 2017, 44(12): 3426-3433.
[12]	LI Ling, ZHANG Dong-jie, WANG Liang, WANG Xin-xin, CAO Yue, LIU Di. Cloning and Bioinformatics Analysis of FoxN1 Gene of Min Pig [J]. , 2016, 43(4): 928-933.
[13]	XUN Wen-juan, SHI Li-guang, ZHOU Han-lin, HOU Guan-yu, WANG Ding-fa, LI Mao, GUAN Song. Comparative Study on Carcass Traits and Meat Quality of Wuzhishan and Landrace Pigs [J]. , 2013, (5): 93-96.
[14]	ZHANG Dong-jie;LIU Di;WANG Liang;YANG Guo-wei. Expression Changes of Insulin-like Growth Factors (IGFs) System Genes in Cold-induced [J]. , 2012, 39(2): 16-19.
[15]	ZHANG Dong-jie;LIU Di;WANG Liang;BIE Shu;SUN Hong-tao;YANG Guo-wei. Study on Expression of Min Pig Hepafin-binding Epidermal Growth Factor-like Gene in Cold Stress [J]. , 2011, 38(4): 98-100.

Study on Relationship between Intestinal Epithelium Tight Junction Proteins mRNA Expression and Piglets Weaning Diarrhea

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments