猪呼吸道疾病综合征中性粒细胞cAMP-PDE和血浆cAMP、IL-6、TNF-α的变化及中药疗效研究

Abstract

Abstract: The study observed the variety of cyclic AMP phosphodiesterase (cAMP-PDE) activity in polymorphonuclear neutrophils (PMN) and contents of cAMP,IL-6,TNF-α in plasma of porcine respiratory disease complex (PRDC),the therapeutic effect and influence of self-made Chinese herbal decoction,for accumulating information of respiratory inflammation pathological mechanism and its prevention.Divided pigs with PRDC into disease group and treatment group, and normal ones as control group. Drenched treatment group with 1 g crude drug/mL Chinese herbal decoction, which was composed of reed rhizome,mongolian milkvetch root,white peony root and liquorice root,twice a day and 1.5 mL/kg body weight for ten days. Observed changes of the number of white blood cell in peripheral blood,neutrophils were isolated from whole blood,HPLC was used to test its cAMP-PDE activity and ELISA kit was used to test the contents of IL-6,TNF-α in plasma.Compared with control group,the number of white blood cell in disease group increased significantly (P<0.05),cAMP-PDE activity and contents of TNF-α,IL-6 increased extremely significantly (P<0.01);but TNF-α in treatment group increased significantly (P<0.05). Compared with the disease group,the number of white blood cell,cAMP-PDE activity and content of TNF-α in treatment group decreased significantly (P<0.05), content of IL-6 decreased extremely significantly (P<0.01).The results showed that abnormal inflammatory reaction existed in PRDC,cAMP-PDE activity,the contents of IL-6 and TNF-α,the number of white blood cell increased significantly.Self-made Chinese herbal decoction could improve the clinical symptoms of the disease,of which the function mechanism was related to the reduction of the white blood cell number,inhibition of cAMP-PDE activity,and reduction of contents of IL-6 and TNF-α in plasma.

Key words: porcine respiratory disease amplexc (PRDC); polymorphonuclear neutrophils (PMN); cyclic AMP phosphodiesterasec (cAMP-PDE); IL-6; TNF-α; Chinese herbal medicine

CLC Number:

Q592.1

ZHANG Mei-hua, LI Jia, MENG Le, ZHOU Shuang-hai, JIANG Dai-xun, CHEN Wu. Study on Cyclic AMP Phosphodiesterase in Polymorphonuclear Neutrophils and cAMP,IL-6,TNF-α in Plasma of Porcine Respiratory Disease Complex and the Therapeutic Effect of Chinese Herbal Medicine[J]. , 2014, 41(9): 132-135.

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Study on Cyclic AMP Phosphodiesterase in Polymorphonuclear Neutrophils and cAMP,IL-6,TNF-α in Plasma of Porcine Respiratory Disease Complex and the Therapeutic Effect of Chinese Herbal Medicine

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