罗汉松内生真菌的抑菌活性研究

Abstract

Abstract: In order to study whether the secondary metabolites of endophytic fungi had antibacterial activity or not, 96 endophytes were isolated from P.macrophyllus in Guizhou province. The antibacterial activity of chloroform/methanol extracts of fermentation broth for 96 fungi was determined by cylinder plate method. 49% endophytic fungi from P.macrophyllus exhibited antibacterial activities against at least one bacterium among three pathogenic bacteria, Escherichia coli, Bacillus subtilis or Staphylococcus aureus. Out of the fungal endophytes isolated from P.macrophyllus, the extracts with 100 folds dilution of strain LHS18, LHS42, LHS67, LHS92 and LHS93 still displayed strong inhibitory activities against S.aureus and strain LHS30 against E.coli. Two strains, LHS18 and LHS42, were further identified to be Aspergillus fumigatus, LHS92 to be Neurospora tetrasperma, LHS30 to be Aphanocladium sp., LHS67 to be Phomopsis liquidambari, respectively. LHS93 was not identified for the low identity of internal transcribed spacer (ITS) sequence between rRNA genes with the known sequences of fungi in Nucleotide database. It suggested that 49% of the endophytic fungi isolated from P.macrophyllus showed potent antibacterial activity, which could be a resource for new antibiotics in future.

Key words: Podocarpus macrophyllus; medicine plant; endophyte; antibacterial activity

RAN Xue-qin, HE Ying-xia, SUN Duan-fang, YE Jing, WANG Jia-fu. Antibacterial Activity of Endophytic Fungi from Podocarpus macrophyllus[J]. .

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Antibacterial Activity of Endophytic Fungi from Podocarpus macrophyllus

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