脂多糖对猪繁殖与呼吸综合征病毒诱导的抗病毒免疫反应的影响

Abstract

Abstract: To study the effects of lipopolyscaccharide on antiviral immune response induced by porcine reproductive and respiratory syndrome virus (PRRSV), porcine alveolar macrophage (PAM) cell was prepared and cultured with PRRSV solution 100 TCID₅₀. After inoculated 12 h, cells were treated with 100 ng/mL LPS. Then collected cells and supernatants after treated 12, 24, 36 and 48 h, respectively. The level of the virus, IFN-α and TNF-α mRNA in the PAM cells were quantitated by Real-time qPCR including lipopolysaccharide (LPS) stimulation of PRRSV infected PAM cells group, PRRSV infection control group, LPS stimulated cells control group and cell control group. The results showed that compared with unstimulated with LPS, the replication of PRRSV in PAM cells infected with PRRSV stimulated with LPS group was less than that of PRRSV infection control group from 12 to 48 h. The transcription level of IFN-α mRNA improved significantly （P＜0.05） and the transcription level of TNF-α mRNA improved and reduced at 48 h. Compared with LPS stimulation control group, LPS stimulation of PRRSV infected PAM cells group, IFN-α mRNA transcription level improved at 12 h and reduced at 24, 36 and 48 h. So PRRSV infected PAM cells stimulated with LPS, the transcription of IFN-α, TNF-α mRNA was significantly improved （P＜0.05）, thereby inhibiting the replication of PRRSV in PAM cells.

Key words: porcine reproductive and respiratory syndrome virus; porcine alveolar macrophage; IFN-α; TNF-α; lipopolysaccharide

WANG Dong-mei, FENG Ya-qiong, CUI Chun-xiao, LI Na-na, DU Dong-ying, QIN Yun-jie, FU Peng-fei, XIA Ping-an. Effects of Lipopolysaccharide on Antiviral Immune Response Induced by Porcine Reproductive and Respiratory Syndrome Virus[J]. .

References

1 张宜娜，李珂，周永辉，等. 免疫复合物对猪繁殖与呼吸综合征病毒诱导的PAM细胞因子转录的影响［J］. 畜牧兽医学报，2012，43(10)：1589～1597.
2 徐红运，夏平安，王中明，等. 猪肺巨噬细胞FcyR Ⅲ受体基因的克隆与序列分析［J］. 中国畜牧兽医，2010，37(4)：66～71.
3 Ait-Ali T, Wilson A D, Carré W, et al. Host inhibits replication of European porcine reproductive and respiratory syndrome virus in macrophages by altering differential regulation of type-Ⅰ interferon transcriptional response［J］. Immunogenetics, 2011, 63(7): 437～448.
4 An H, Xu H， Zhang M, et al. Src homology 2 domain-containing inositol-5-phosphatase 1 (SHIP1) negatively regulates TLR4-mediated LPS response primarily through a phosphatase activity- and PI-3K-independent mechanism［J］. Blood, 2005, 105(12): 4685～4692.
5 Beyer J, Fichtner D, Schirrmeier H, et al. Porcine reproductive and respiratory syndrome virus (PRRSV): Kinetics of infection in lymphatic organs and lung［J］. Vet Med B Infect Dis Vet Public Health, 2000, 47(1): 9～25.
6 Genini S, Delputte P L, Malinverni R, et al. Genome-wide transcriptional response of primary alveolar macrophages following infection with porcine reproductive and respiratory syndrome virus［J］. General Virology, 2008, 89(10): 2550～2564.
7 Goto A, Matsuahita K, Gesellchen V, et al. Akirins are highly conserved nuclear proteins required for NF-κB-dependent gene expression in drosophila and mice［J］. Nat Immunol, 2008, 9(1): 97～104.
8 Khattabi E I, Adams M H, Heezins E, et al. Llama single-chain antibody that blocks lipopolysaccharide binding and signaling: Prospects for therapeutic applications［J］. Clin Vaccine Immunol, 2006, 13(10): 1079～1086.
9 Kim J J, Ayyavoo V, Bagarazzi M L, et al. In vivo engineering of a cellular immune response by coadministration of IL-2 expression vector with a DNA immunogen［J］. Immunol, 1997, 158(2): 186～826.
10 Kojawski L A, Talpaz M. The role of inteferon-alpha in the treatment of chronic myeloid leukemia［J］. Cytokine Growth Factor Rev, 2007, 18(5～6): 459～471.
11 Kuwata H, Watanabe Y, Miyoshi H, et al. IL-10-inducible Bcl-3 negatively regulates LPS-induced TNF-alpha production in macrophages［J］. Blood, 2003, 102(12): 4123～4129.
12 Loving C L, Brockmeier S L, Sacco R E. Differential type Ⅰ interferon activation and susceptibility of dendritic cell populations to porcine arterivirus［J］. Immunology, 2006, 120(2): 217～229.
13 Manoir J M, Albright B N, Stevenson G, et al. Variability of neutrophil and pulmonary alveolar macrophage function in swine［J］. Vet Immunol and Immunopathol, 2002, 89(3～4): 175～186.
14 Micheau O, Tschopp J. Induction of TNF receptor Ⅰ-mediated apoptosis via two sequential signaling complexes［J］. Cell, 2003, 114(2): 181～190.
15 Van G H, Van B W, Peter L, et al. Sialoadhesin and CD163 join forces during entry of the porcine reproductive and respiratory syndrome virus［J］. Gen Virol, 2008, 89(2): 2943～2953.
16 Yamamoto M, Sato S, Hemmi H, et al. Role of adaptor TRIF in the MyD88-independent toll-like receptor signaling pathway［J］. Science, 2003, 301(5633): 640～643.
17 Yan Z, Zhao N, Wang Z, et al. A mutated human tumor necrosis factor-alpha improves the therapeutic index in vitro and in vivo［J］. Cytotherapy, 2006, 8(4): 415～423.

[1]	ZHONG Yueyun, YANG Shuzhan, CHEN Feiyue, LU Zhier, LI Bingxin, LI Wanyan, TIAN Yunbo, HUANG Yunmao, XU Danning, CAO Nan. Effects of LPS on Apoptosis of Goose Embryo-derived Hepatocytes ROS/p38/MAPK Signaling Pathway [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2224-2232.
[2]	KANG Pu, REN Zhaowen, CAI Rujian, LYU Zongji, HUANG Yuhao, WANG Xiaohu. Variation Regularity and Transmission of PRRSV-2 Lineage 8 Strains in Guangdong and Surrounding Provinces [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2001-2011.
[3]	CHEN Tianbao, WANG Yani, LIU Baoling, WANG Gang, WANG Xiaohu, LIU Xuhui, CAI Rujian, HE Dongsheng. Research Progress on the Anti-Porcine Reproductive and Respiratory Syndrome Virus Effect and Mechanism of Traditional Chinese Medicine [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2114-2129.
[4]	SHAO Yongbin, ZHENG Yichuang, ZHANG Jingyi, WEI Linying, TIAN Yong, SUN Guojie, LIU Yuran, LUO Yan, GU Xinli. Study on the Anti-inflammatory Effect of Leonurine on LPS-induced Endometritis in Mice [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2149-2155.
[5]	LI Huawei, GUO Kewei, WANG Xuying, ZHANG Xiaoyu, LU Ziwei, LI Xinfeng, MA Hui, HOU Wenjing. Eukaryotic Expression and Subcellular Localization of Nonstructural Protein 7 of Porcine Reproductive and Respiratory Syndrome Virus [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1160-1168.
[6]	FU Jingcheng, HE Junhui, TONG Jiang, WU Wei, GUO Shuang, YANG Yanbin, HAN Yingqian, WANG Yueying, LI Heping. Anti-inflammatory and Antioxidant Roles of HO-1 in Macrophages [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 807-816.
[7]	GAO Jiahao, QIAO Yanjie, LIAN Kexun, WANG Mengmeng, GU Xinli, SHAO Yongbin. Effects of Glycyrrhizic Acid on Immune Performance of Lipopolysaccharide-stressed Yellow Feather Broilers [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3419-3427.
[8]	TANG Lin, ZHANG Junfang, WANG Ying, SUN Bin, WANG Enze, SHIN Jongsuh, GUO Panpan, JIN Xin, YAN Changguo, LI Xiangzi, LI Qiang. Anti-apoptotic Effect of Canine Adipose-derived Mesenchymal Stem Cells on Endoplasmic Reticulum Stress Model of Severe Acute Pancreatitis in vitro [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1840-1851.
[9]	ZHANG Yuzhu, YU Chao, ZHENG Huihua, TANG Xinyue, HUANG Ronglei, XIE Guanghong. Mechanism Study of Protocatechuic Acid on LPS-induced Endometritis in Mice [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1585-1591.
[10]	LIU Qingqing, WU Peng, LI Peidong, ZHANG Jiangwei, CHEN Chuangfu, XIAO Chencheng. Construction of Recombinant Adenovirus Expressing Porcine Reproductive and Respiratory Syndrome Virus N Protein and Its Immunogenicity Analysis [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 1024-1031.
[11]	WANG Zhaoli, YANG Siyu, WU Yanmei, SONG Xiaoxiao, MU Xiang, ZHANG Tao. Effects of PRRSV-LPS Treatment on Neutrophil Adhesion to Porcine Pulmonary Microvascular Endothelial Cells [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 471-479.
[12]	YANG Yu, GUAN Wenchao, CUI Xiaozhen, JIA Yongxin, YANG Shiyu. Effects of Licochalcone A on Oxidative Stress Induced by Haemophilus parasuis Infection in Porcine Alveolar Macrophages [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 480-487.
[13]	SUN Lu, ZHANG Jing, YIN Miao, CHENG Yaodie, DAI Kun, WU Qian, ZHAO Hong, CHEN Xiwen. Study on the Antibacterial and Anti-PRRSV Activities of Four Heat Clearing and Detoxifying Compound Chinese Herbal Medicine in vitro [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 746-754.
[14]	CHU Pinpin, JIANG Zhiyong, LI Chunling, GOU Hongchao, LI Yan, CAI Rujian. Antiviral Effect of Silver Nanoparticles on Porcine Reproductive and Respiratory Syndrome Virus in vitro [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(11): 4356-4363.
[15]	MA Sijia, LIU Yuan, WANG Xuzhong, LI Tingting, DUAN Xing, YANG Songbai, SONG Dan, LI Xiangchen. Effects of Melatonin on the Expression of Inflammatory Factors in Skeletal Muscle Satellite Cells Stimulated by LPS in Tan Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(9): 3378-3386.

Effects of Lipopolysaccharide on Antiviral Immune Response Induced by Porcine Reproductive and Respiratory Syndrome Virus

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments