葡萄原花青素活性组分的分离及其对肉仔鸡外周血淋巴细胞增殖的影响

Abstract

Abstract: This experiment was conducted to study the effect of grape procyandins （GPC） and its ingredients on the proliferation of peripheral blood lymphocytes （PBLs）of broiler，and to develop the method to screen active ingredients of GPC.Six concentrations （0，0.24，1.2,6.0,30,150 μg/mL） of GPC were chosen to study the best concentration on the proliferation of PBLs of chickens.RT-HPLC was used to gotten chromatograms of GPC，different ingredients were gotten by Real-time fractionation and different ingredients in the best concentration were used to get the best one.The result showed that the best concentration of GPC was 30 μg/mL and the best ingredient fraction was 16 to 25 min.

Key words: grape procyanidins; broiler; peripheral blood lymphocytes; HPLC; ingredients

YANG Jin-yu，WANG Jing，ZHANG Hai-jun，WU Shu-geng，YUE Hong-yuan，QI Guang-hai. Separation of Active Ingredients in Grape Procyanidins and its Effects on Proliferation of Peripheral Blood Lymphocytes in Broiler[J]. .

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Separation of Active Ingredients in Grape Procyanidins and its Effects on Proliferation of Peripheral Blood Lymphocytes in Broiler

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