鸡鲍氏3型志贺菌脂多糖及O抗原多糖的提纯与鉴定

Abstract

Abstract: To obtain LPS and its subunit O-PS with high purity from chicken S.boydii type 3, LPS from chicken Shigella was extracted with modified hot phenol-water method, high purified LPS was obtained through sephadex gel chromatography, O-PS was obtained by combination with acid hydrolysis method and sephadex gel chromatography. Biochemical assay showed that the purified LPS and O-PS with high purity, rabbit ideal loop experiment demanstrated that there was high activity in purified LPS.The result indicated that extracted LPS and O-PS could be used in specific pathogenesis research and developed as effective vaccine candidates.

Key words:

chicken S.boydii type 3; LPS; Omso-bidi-font-family: 宋体" lang="EN-US">-PS; purification

WEI Ya-peng,GUO Hui-juan,ZHANG Mei-ling,CHANG Hong-tao,LIU Hong-ying, WANG Chuan-qing,WANG Xin-wei,YANG Xia,CHEN Lu,ZHAO Jun. Extraction and Identification of Lipopolysaccharide and O-polysaccharide from Chicken S.boydii Type 3[J]. China Animal Husbandry & Veterinary Medicine.

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Extraction and Identification of Lipopolysaccharide and O-polysaccharide from Chicken S.boydii Type 3

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