贵州本地山羊线粒体转录因子B2基因5′调控区SNP筛查与生物信息学分析

Abstract

Abstract: In order to screen the single nucleotide polymorphism （SNP） sites in the promoter region of mitochondrial transcription factor B2 （TFB2M） gene in goat and analyze the effect of SNP on function elements of promoter, three goat breeds (Qianbei Ma goat, Guizhou Black goat and Guizhou White goat) were selected to construct DNA pools, SNP sites were screened by direct sequencing subsequently. 4 SNPs which included G-601C, C-381A, C-286T and G-283T respectively were found in the 5′ flanking region of TFB2M gene. Furthermore, bioinformatics tools were used to predict the core region of the promoter and CpG island. It demonstrated that various novel transcription factors binding sites emerged while a large amount of transcription factors binding sites existed before disappeared based on the SNPs found in this study, while it didn’t affect the range of CpG island dramaticlly by using various softwares. There were four SNPs existed in the 5′ flanking region of TFB2M gene that had important effects on promoter elements. This work could lay the experimental foundation for the identification of function of TFB2M promoter.

Key words: TFB2M gene; SNP; promoter; Qianbei Ma goat; Guizhou White goat; Guizhou Black goat

XIE Hai-qiang, LIU Ruo-yu, GONG Yu, JIAO Ren-gang. SNP Screening and Bioinformatics Analysis of 5′-flanking Region of TFB2M Gene in Local Goat in Guizhou Province[J]. .

References

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SNP Screening and Bioinformatics Analysis of 5′-flanking Region of TFB2M Gene in Local Goat in Guizhou Province

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