鸡源大肠杆菌超广谱β-内酰胺酶的检测及耐药性分析

Abstract

Abstract: In order to investigate extended spectrum beta-lactamases (ESBLs) production and drug resistance of Escherichia coli strains isolated from chickens in Taizhou areas, 13 strains of Escherichia coli isolates were tested. ESBLs were examined and minimum inhibitory concentrations (MICs) of different drugs against ESBLs producing strains and non-ESBLs-producing strains were detected by broth microdilution method. The results showed that 5 out of the total 13 isolates were identified as ESBLs producing strains, and the positive rate was 38.5%. And drug resistance of ESBLs producing strains to β-lactam antibiotics was extremely serious with apparently higher drug-resistance rate than those of the non-ESBLs-producing strains, resistance rate of the ESBLs producing strains and non-ESBLs-producing strains to ampicillin were 100% and 62.5%, respectively; resistance rate to ceftiofur were 100% and 12.5%, respectively; resistance rate to ceftriaxone were 80% and 20%, respectively. To other antimicrobial resistance rates were apparently higher than those in non-ESBLs-producing strains.Ampicillin and shubatan (2:1) combined with the single ampicillin compared to ESBLs producing strains MIC value reduced to single ampicillin at 1/16 to 1/8, whereas no significant changes happened on non-ESBLs-producing strains. Ampicillin and Amikacin (1:1)combined compared to any kind of both, on ESBLs producing bacteria and non-ESBLs-producing strains MIC values were reduced to a single drug 1/16 to 1/2.The results showed that, the clinica choice of mixed preparation of betalactam and beta lactamase inhibitors to treat ESBLs producing chicken Escherichia coli infection, the drugs of different kinds or different mechanism were also combincated to treat chicken Escherichia coli infection, especially drug resistance chicken Escherichia coli infection.

Key words: Escherichia coli; extended spectrum beta-lactamases (ESBLs); drug resistance

CLC Number:

S852.61

LIU Yu-hua, WANG Lin-lin, FANG Xiang-hong, DAI Li-hong. Detection and Analysis of Drug Resistance of ESBLs in Escherichia coli Strains Isolated from Chickens[J]. , 2014, 41(12): 271-275.

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Detection and Analysis of Drug Resistance of ESBLs in Escherichia coli Strains Isolated from Chickens

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