河南省某地区健康人源及动物源肠球菌种属分布及耐药性差异研究

Abstract

Abstract: The assay was aimed to evaluate the differences of prevalence and drug resistance of Enterococcus species among human, broilers and swines in Henan province, China. Totally 220 stool samples were collected, and identified by 16S rDNA sequence analysis and the Rapid ID 20 Strep System. There were significant differences among the total isolation rate of Enterococcus, isolation rate of E.faecalis, isolation rate of E.faecium in 3 different origins (P<0.05).A total of 156 (70.91%) Enterococcus spp. isolates were recovered. The isolation rate of Enterococcus from swine origin was the highest (86.00%), while human origin was the lowest (62.63%), There were significant differences between the isolation rates of swine and human origns (P<0.018). The most predominant specie was E.faecium (31.36%) in human, while in broilers and swines were E.faecalis (28.17% and 32.00%, respectively). All isolates were sensitive to vancomycin and teicoplanin. The resistant rate of many multidrug-resistant (MDR) of the isolates from human, broilers and swines were statistically significant (P<0.05).The resistant rates of isolates from human origin Enterococcus spp.to erythromycin (69.35%), ciprofloxacin (37.10%) and ampicilin (19.35%) were higher than those from broiler origin and swine origin. The isolates resistant rates to tetracycline (88.24%), florfenicol (11.76%) and chloramphenicol (21.57%) from broiler origin Enterococcus spp. were higher than those from the other two origins, respectively. The MDR rates of human (35.48%) and broiler (30.19%) origins isolates were higher than swine origin (7.84%). The observation in this study suggested that the prevalence and drug resistance of Enterococcus spp. from human, broilers and swines in Henan province were different, and the MDR isolates were prevalent in various origins Enterococcus. More attention should be given on investigation and research of the resistance of Enterococcus among human and animals to better understand the epidemic of the antidrug-resistant Enterococcus and minimize the spread of it effectively.

Key words: Enterococcus spp.; isolation rate; drug resistance; difference

CLC Number:

S852

LIU Jia, CHEN Xia, ZHAO Ai-lan, YUAN Min, XIONG Yan-wen, GONG Lin, LU Jin-xing, LI Juan, LIANG Jian-qin. Study on the Differences of Prevalence and Drug Resistance of Enterococcus Species of Human and Animal Origins in Henan Province, China[J]. , 2014, 41(12): 172-177.

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Study on the Differences of Prevalence and Drug Resistance of Enterococcus Species of Human and Animal Origins in Henan Province, China

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