miR-138对小鼠乳腺发育及泌乳功能的影响

Abstract

Abstract: microRNAs (miRNAs) play a key role in cell proliferation,differentiation and apoptosis. miRNA involved in the regulation of mammary gland development in cell proliferation and differentiation. In this experiment, we used mice as an animal model to study the role of miR-138 in mouse mammary gland development and lactation. Maternal tail vein injection of miR-138 inhibitors, pups weighing method applied to detect the changes of milk yield;electron microscopy techniques was used to observe ultrastructure changes in mammary gland tissue. The results showed that after intravenous injection of miR-138 inhibitor, female mouse increased maternal mammary epithelial cells, increasing the amount of milk secretion,ultrastructure of cells found in the metabolic activity,collect female mouse milk after miR-138 inhibitor, detect lactose and casein of the milk were all increased. The results suggested that the inhibition of miR-138 could stimulate mammary epithelial cell proliferation, increase the secretion of milk, and regulation of the ingredient of the milk.

Key words: miR-138; mammary gland development; lactation

CLC Number:

LI Dan, WANG Jie, XIE Xue-jiao, LI Qing-zhang, WANG Chun-mei. Effect of miR-138 on Development and Lactation in Mouse Mammary Gland[J]. , 2014, 41(11): 223-227.

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Effect of miR-138 on Development and Lactation in Mouse Mammary Gland

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