水牛PNPLA8基因编码区克隆、表达分析及催乳素对该基因的调节作用

doi:10.16431/j.cnki.1671-7236.2020.09.003

Abstract

Abstract: In order to investigate the role of patatin-like phospholipase domain-containing 8(PNPLA8) in lipid metabolism of mammary gland in buffalo,the coding region (CDS) was amplified and cloned by PCR based on the sequence of Bos taurus PNPLA8 gene in GenBank (accession No.:XM_005205444.4) were analyzed using bioinformatics software.Total RNA was extracted from different tissues of buffalo and mammary glands,which were harvested from different lactating buffaloes.The expression of PNPLA8 gene mRNA in different tissues and different lactation period was detected by Real-time quantitative PCR.For buffalo mammary epithelial cell treatment,different concentrations of prolactin were used and the effect of prolactin on the expression of PNPLA8 gene was detected by Real-time quantitative PCR.The results showed that the length of the PNPLA8 gene CDS was 2 355 bp,encoding 784 amino acids.The sequence showed high homology with Bos mutes and Capra hircus.PNPLA8 gene was expressed at different levels in 11 tissues examined,with a relatively high level in the lung and mammary tissues while the low level in the fat and muscle tissues.The expression abundance of the PNPLA8 gene was variable during lactation and showed a trend of "low-high-low".Prolactin treatment showed that the expression of PNPLA8 gene decreased with the increase of prolactin concentration.In this study,PNPLA8 gene of buffalo was successfully cloned,and the expression of PNPLA8 gene in different tissues and the lactation period was analyzed.Herein,the effect of prolactin on the expression of PNPLA8 gene was studied that laid a foundation for further research on PNPLA8 gene of mammary gland in buffalo.

Key words: buffalo; PNPLA8 gene; cloning; lactation; mammary epithelial cell

CLC Number:

S829.9

MA Xiaoya, PANG Chunying, LU Xingrong, DUAN Anqin, LIANG Shasha, LIANG Yanyin, DENG Tingxian, LIANG Xianwei. Cloning,Expression Analysis and Regulation of PNPLA8 Gene by Prolactin in Buffalo[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(9): 2715-2723.

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Cloning,Expression Analysis and Regulation of PNPLA8 Gene by Prolactin in Buffalo

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