日粮精粗比对活体外瘤胃发酵和烟酸、烟酰胺合成的影响

Abstract

Abstract: The gas production procedure was conducted to determine the effect of different concentrate to forage ratio on rumen fermentation parameter and rumen microbial synthesis of niacin,niacinamide (NAM),and nicotinic acid (NA) in vitro.Rumen fluid was collected from four Holstein adult steer with permanent rumen fistula,and three dietary substrates with different concentrate to forage ratios (30:70,50:50,70:30,DM) at an equal crude protein level of 13.5% were formulated by adjusting inclusion levels of starch,avicel and casein.The results showed that with the concentrate to forage ratios increased,the in vitro rumen pH,molar percentages of acetic and acetate to propionate were extremely significantly decreased (L,P<0.01),the ammonia-N concentration was significanthy decreased (L,P<0.05),whereas the 72 h gas production,molar percentages of propionate,butyrate and total VFA were extremely significantly increased (L,P<0.01).With the dietary concentrate to forage ratios increased,the concentrations of total niacin (nicotinic acid+niacinamide),niacinamide were increased at 24 and 48 h (L,0.05<P<0.10),while the concentration of nicotinic acid had no evident changing tendency (P>0.10).In conclusion,dietary concentrate to forage ratio had a greater effect on rumen fermentation parameters and synthesis of niacin,niacinamide,and as dietary concentrate to forage ratios increased,the concentrations of total niacin and niacinamide had an increasing tendency.

Key words: dietary concentrate to forage ratio; rumen fermentation; niacin; niacinamide; synthesis

CLC Number:

S816

ZHAO Yun-jun, MENG Qing-xiang, ZHANG Jun-yu, GAO Li, YAO Fang. Effect of Dietary Concentrate to Forage Ratio on Rumen Fermentation and Microbial Synthesis of Niacin,Niacinamide in vitro[J]. , 2014, 41(11): 101-106.

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Effect of Dietary Concentrate to Forage Ratio on Rumen Fermentation and Microbial Synthesis of Niacin,Niacinamide in vitro

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