抗猪瘟病毒单克隆抗体的制备及特性鉴定

Abstract

Abstract: 4 SPF BALB/c mice were immunized with purified classical swine fever virus (CSFV) as the antigen and mouse splenic cells were fused with SP2/0 cells. Hybridoma cells were screened by ELISA and semi-solid agar cloning, 4 hybridoma cells secreting anti-CSFV monoclonal antibodies were obtained. ELISA results showed that all of these monoclonal antibodies with high antibody titer, which could specifically react to CSFV but not cross react to other pig viruses and PK-15 cell cultures. Western blotting indicated that 3 strains of monoclonal antibodies reacted with CSFV specially. Specific green fluorescence was observed in the cell membrane by IFA. This study layed a foundation for the development of new diagnostic reagents for CSFV.

Key words: classical swine fever virus (CSFV); monoclonal antibody; preparation; identification

CLC Number:

S813.2

WANG Ai-hua, XU Ke-jun, WANG Jin-liang, WEI Feng, SUN Quan-wen. Preparation and Characteristic Identification of Monoclonal Antibodies against Classical Swine Fever Virus[J]. , 2014, 41(11): 97-100.

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Preparation and Characteristic Identification of Monoclonal Antibodies against Classical Swine Fever Virus

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