Abstract: The purposes of this study was to checking the security and availability of Chinese herbal medicines on Aeromonas veronii disease of Acipenser baerii.There were three separated experiments in the study.Security trial:5000 mg/kg·BW Chinese herbal medicines coarse powder (CP) and superfine powder (SP) were drenched to 10 fish per repeat in six repeat groups,respectively.The mortality was recorded for 1 week.Preventive trial:The control fish group was fed with basic feed,and CP and SP fish group were fed with feed including 5% CP and SP,respectively,each group had 3 repeats with 15 fish in each repeat.After two treatment feeding period,all fish were challenged with intramuscular injecting Aeromonas veronii.The mortality was recorded for 10 days.Pilot-scale test:The control fish group was fed with basic feed,and CP and SP fish group were fed with feed including 5% CP and SP,respectively,each group had 3 repeats with 25 fish/m² per repeat.After two treatment feeding period,3 fish in each repeat were randomly taken blood samples from tail vein to detect non-specific immune-related index.The results showed that LD₅₀ of both CP and SP herbs to Acipenser baerii were more than 5000 mg/kg·BW.The results of preventive trial indicated that challenging survival rate of CP and SP groups were significantly higher than that of control group (P<0.05).The results of pilot-scale test showed that the level of SOD in CP group was significantly higher than that in control group (P<0.05),the level of MDA and C3 in SP group were significantly higher than those in CP and control groups (P<0.05),the level of AKP in SP group was significantly higher than that in control group (P<0.05).In conclusion,the Chinese herbal medicines had significantly prevention effects on Aeromonas veronii disease of Acipenser baerii,superfine powder of the Chinese herbal medicines could improve the non-specific immunity of Acipenser baerii.

Key words: Chinese herbal medicine; Acipenser baerii; bacterial disease resistance; herbal particle size