牛病毒性腹泻病毒BVDV-JL分离株全基因序列测定与分析

Abstract

Abstract: The bovine viral diarrhea virus (BVDV) JL strain was isolated from a cattle showing clinical diarrhea signs in Jilin province. In order to analyze the complete genome of BVDV-JL,7 fragments of BVDV-JL were amplified by RT-PCR and cloned into pMD18-T vector then sent for sequencing by commercial serves. Complete genome of the virus was 12276 bp in length and contains a 5'UTR,one open reading frame encoding a polyprotein of 3901 amino acids and a 3'UTR. The results of genomic comparison and phylogenetic analysis showed that BVDV-JL fell into BVDV-1b2 subtype and was most similar to CP7,the nucleotide was 93.2%. BVDV-JL was the first reported BVDV-1b2 strains,BVDV-JL complete genomic sequences enriched the available genomic sequences and it would be benefit to epidemiological surveys of BVDV detected in China.

Key words: bovine viral diarrhea virus; complete genome; phylogeny

CLC Number:

S852.65

ZHANG Shu-qin, TAN Bin, WANG Feng-xue, CHENG Shi-peng. Complete Genome Sequence Analysis of Bovine Viral Diarrhea Virus BVDV-JL in China[J]. , 2014, 41(10): 23-27.

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Complete Genome Sequence Analysis of Bovine Viral Diarrhea Virus BVDV-JL in China

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