体细胞核移植生产梅山猪

Abstract

Abstract: In this study,Meishan pigs of Chinese excellent local varieties was used as materials,the pig fetal fibroblasts was obtained by pancreatic enzyme digestion method,SRY-1 and SRY-2 were used as primers,though PCR identification,male fetus samples could amplify out 250 bp of Y chromosome specific genes,while the female fetus samples could not amplify the stripe. The fusion efficiency of nuclear transfer embryo using G1,G2,G3,G4 and G5 generation as a donor cell had no significant difference (P>0.05),but the cleavage rate by SCNT using G5 generation cell was significantly higher than G1,G2 and G3 and G4 (P<0.05). Used the G5 generation of fetal fibroblasts of Meishan pigs as donor cells,we successfully cloned a Chinese Meishan pig by SCNT,and the piglets were all male fetus. The results showed that the method of sex identification was not only the easy operation,but also high accuracy,the analysis of microsatellite genotyping for Meishan pigs confirmed that the piglets were genetically derived from fetal fibroblasts of Meishan pigs and unrelated to their surrogate mother. This study will provide a effective feasible method for pig breeding,gender control,and human disease models,and lay a foundation for mass production of cloned excellent breeding with high productivity.

Key words: Meishan pigs; cloned piglets; somatic cell nuclear transfer; pig fetal fibroblasts

CLC Number:

Q813

HUA Zai-dong, ZHENG Xin-min, WEI Qing-xin, XIAO Hong-wei, LIU Xi-mei, HUA Wen-jun, GUO Shuai, ZHANG Li-ping, BI Yan-zhen, REN Hong-yan, CEN Wen, HUA Sheng. Production of Cloned Meishan Piglets by Somatic Cell Nuclear Transfer[J]. , 2014, 41(10): 192-197.

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Production of Cloned Meishan Piglets by Somatic Cell Nuclear Transfer

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