检测马疱疹病毒4型抗体间接ELISA方法的建立及应用

Abstract

Abstract: In order to establish an indirect ELISA method for detection of equine herpesviruses type 4 （EHV4） antibody, the glycoprotein G （gG） protein with specific epitope worked as a detection antigen. After optimizing conditions, the indirect ELISA method was developed successfully，specificity and repeatability tests were determined. The result was that the EHV4 gG only reacted with antibodies against EHV4;but not with antibodies against EHV1; both the intro-batch and inter-batch variation coefficiencies were lower than 10%.Concordance of the indirect ELISA relative to commercial EHV1/4 antibody kit was above 90%.The results indicated that the indirect ELISA method could be used for the detection and epidemiological surveys of EHV4 infection.

Key words: detection; equine herpesviruses type 4; glycoprotein G; indirect ELISA

TIAN Zhi-ge,GUO Wei,ZHAO Shi-hua,PAN Jia-liang,RAN Duo-liang, XIANG Wen-hua,QU Juan-juan. Establishment and Application of ELISA for Detection of Equine Herpesviruses Type 4 Antibodies[J]. China Animal Husbandry & Veterinary Medicine.

References

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Establishment and Application of ELISA for Detection of Equine Herpesviruses Type 4 Antibodies

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