库拉索芦荟提取物对维氏气单胞菌抑菌作用的研究

Abstract

Abstract: To discuss the bacteriostasis of extracts from aloe vera on Aeromonas veronii by juicing method, water extraction and alcohol extraction method, the inhibition activities, the minimal inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of the extracts from three kinds of methods which had significantly antibacterial effect against Aeromonas veronii were determined with K-B programe and the double broth dilution method. The results showed that the extracts from aloe vera with three kinds of methods all displayed sterilization ability on Aeromonas veronii inordinately. MIC of the extracts from aloe vera with juicing method, water extraction and alcohol extraction method were 1, 0.5, 0.125 g/mL, respectively. The MBC were 1, 1, 0.25 g/mL, respectively. Alcohol extract method showed higher activities than the others. But water extraction was considered as the most suitable method for extracting based on both of the antifungal activity and extraction efficiency.

Key words: aloe vera; extractive; Aeromonas veronii; antibacterial effect

CLC Number:

YANG Yong-li, WANG Li, WU Man-lin. Study on the Bacteriostasis of Aloe Vera Extracts against Aeromonas veronii[J]. , 2013, 40(9): 147-150.

References

1 丁汀.抗生素在水产养殖应用中的利和弊[J].华夏星火,2003(10):43.
2 马志宏,李铁梁,姜娜,等.中草药对致病性维氏气单胞菌体外抑菌活性及最优组方研究[J].中国畜牧兽医,2011,38(6):155~159.
3 王锦旭,王鑫,叶敬波,等.芦荟提取液抗菌效果研究[J].安微农业科学,2008,36(13):5478~5479.
4 古丽斯玛依·艾拜都拉,孜来古丽·米吉提,米合热阿衣·阿不都加帕,等.三种芦荟汁的抑菌活性及其影响因素的研究[J].生物技术,2008,18(5):44~46.
5 刘晓鹏,姜宁,向东山,等.库拉索芦荟多糖超声辅助提取工艺的研究[J].食品科学,2008,29(10):286~289.
6 华春.库拉索芦荟的抑菌作用[J].南京师大学报(自然科学版),2004,27(1):90~93,97.
7 孙振红,魏凯,谭燕玲,等.芦荟粗提物抑菌及抗病毒作用的研究[J].中国预防兽医学报,2011,33(9):694~698.
8 何池义,吴万春,韩真.芦荟的药理作用[J].中国临床药理学与治疗学,2003,8(3):261~264.
9 张美玲,钱和,张根义.大孔吸附树脂分离纯化芦荟苷的研究[J].河南工业大学学报,2002,26(6):57~60.
10 张翠利,李锟,李钦.芦荟中芦荟苷提取工艺研究[J].亚太传统医药,2010,6(8):25~27.
11 张慧君,罗仓学,张新申,等.超声波提取芦荟苦素的研究[J].广州食品工业科技,2004,20(1):15~17.
12 周光,谢骏,周群兰,等.团头鲂养殖池维氏气单胞菌的致病性耐药性及在不同生态位间的差异[J].江苏农业科学,2012,40(5):192~196.
13 晏卫红,黄思量,丘华.芦荟对植物病原真菌生长的抑制效果[J].中国农学通报,2001,17(5):37~40.
14 晏卫红,黄思良,付岗,等.芦荟不同溶剂提取物对白菜黑斑病菌的抑菌活性研究[J].西南农业学报,2009,22(5):1336~1340.
15 祥颖,田义新,李杰.芦荟实用百科[M].长春:吉林科学技术出版社,2000.
16 耿丽晶,周围,王军萍,等.库拉索芦荟皮中果胶提取工艺的优化研究[J].食品工业科技,2012,33(5):101~104.
17 崔红花,赵英日,王淑美.大黄蒽醌类成分的提取方法筛选及均匀设计优化工艺研究[J].中国药房杂志,2010(7):612~614.
18 崔栀,付强,李侠,等.库拉索芦荟抑制内毒素作用的实验研究[J].天津药学,2002(2):38~40.
19 梁立国,邴旭文,张晓君,等.18种中草药煎液对温和气单胞菌的抑、杀菌效果[J].淡水渔业,2010,40(1):66~69.
20 樊黎生.芦荟汁的抗菌作用研究[J].食品与发酵工业,2001,27(8):38~40.
21 黎烔,叶里,卢运新,等.罗非鱼维氏气单胞菌的分离鉴定和药敏实验[J].水生态学杂志,2011,32(3):132~136.
22 Fraisse T,Lechiche C,Sotto A,Lavikne J P. Aeromonas spp infections: Restrospective study in Nimes university hospital,1977-2004[J].Pathologie Biologie,2008,56(2):70~76.
23 Sung H H,Hwang S F,Tasi F M. Responses of giant freshwater prawn (Macrobrachium rosenbergii) to challenge by two strains of Aeromonas spp[J].Journal of Invertebrate Pathology,2000,76(4):278~284.

[1]	SHAO Mingzhu, ZHAO Yunqing, LI Songli, HOU Shaohua, ZHANG Yanying. Isolation and Identification of Escherichia coli from Dairy Cows in Hebei Province and Its Pathogenicity and Antibacterial Effect of Chinese Herbal Medicine in vitro [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3683-3691.
[2]	ZHAO Tianrui, SHAO Jinhua, WANG Chaohao, YAN Rui, LEI Yifei, LIU Xiaoya, HU Changmin. Isolation and Identification of Skin Pathogenic Bacteria from Dogs and Cats and Analysis of Antibacterial Effect of Natural Products [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1556-1567.
[3]	WANG Tianyang, MA Bei, DING Ze, DONG Tianzhen, LIU Junfeng. Preparation and Preliminary Evaluation of Minocycline Hydrochloride Complex Temperature-sensitive Gel [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(12): 4854-4865.
[4]	YIN Junyi, LIU Qiujin, TIAN Qiufeng, WANG Yan, ZHANG Jun, CHEN Nannan, QIN Pingwei, BAI Changsheng, MENG Weishan, SHI Tongrui, GAO Shengyue, SONG Yan, WANG Jiahui, TANG Lingling, HUANG Xuankai, LI Mengshu. Isolation,Identification and Biological Characteristics of a Strain of Lactobacillus casei [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(2): 516-524.
[5]	WANG Hongbin, ZHU Lixia, YANG Nan, WU Tonglei, ZHANG Zhiqiang, GAO Guisheng, SHI Qiumei. Study on Antibacterial Effects of Common Chinese Herbs Against Aquatic Animal Pathogens in vitro [J]. , 2018, 45(5): 1357-1366.
[6]	SHI Feifei, QIN Feng, WANG Xiujun, MA Qilun, GU Shutong, WEI Yalan, LI Yang. Preparation of Florfenicol Nanoemulsion and Observation of Its Antibacterial Effect [J]. , 2018, 45(10): 2936-2942.
[7]	SUN Yan-jie, WU Yong-ji, LIU Zeng-yuan, HU Mei, ZHAO Yu-chuan, HUANG Kai, SI Hong-bin. Antibacterial Effect of Aqueous Extract of Chrysanthemum indicum L.Combined with Antibiotics on Extended Spectrum β-lactamases-producing Escherichia coli [J]. , 2016, 43(8): 2170-2175.
[8]	HE Dong-mei, HU Qiao, TIAN Yu-shi, WEI Yu-qing, XU Jing-wei, CHEN Chao-xi. Study on the Antidiarrhea Effect of Four Kinds of Tibetan Veterinary Drug Prescription [J]. , 2016, 43(7): 1890-1897.
[9]	ZHU Zhi-xiu, JIANG Xin-hua, DENG Shun-zhou, ZHANG Wen-bo, WANG Bei, CHEN Long. Isolation and Identification of Bacillus amyloliquefaciens and Analysis of the Antibacterial Effect against Aeromonas hydrophila [J]. , 2015, 42(3): 734-740.
[10]	ZHENG Yan-qing, ZOU Zhi-ming, HE Jia-kang, SONG Jian-wu, SI Hong-bin. Antibacterial Study of Different Bidens bipinnata L Extracts against Drug-resistant E.coli [J]. , 2014, 41(3): 233-236.
[11]	SHU Gang;ZHAO Xiao-ling;YANG Zhi-qin;RAN Xiao-long;ZHAN Wei;LV Cheng;WANG Kai-yu;. Effects of Extractive from Penthorum chinense Pursh on the Growth Performance and Serum Biochemical Parameters in Broilers [J]. , 2012, 39(4): 94-98.
[12]	LEI Hong-dong;LIANG Jian-ping;GUO Zhi-ting;WANG Xue-hong;HUA Lan-ying. Study on the Acute Toxicity of Dichroa Febrifuga Extractive [J]. , 2011, 38(6): 236-238.

Study on the Bacteriostasis of Aloe Vera Extracts against Aeromonas veronii

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 12

Recommended Articles

Metrics

Comments