氟苯尼考纳米乳制备及其抑菌效果观察

doi:10.16431/j.cnki.1671-7236.2018.10.032

Abstract

Abstract:

In order to successfully prepare florfenicol nanoemulsion and investigate its bacteriostatic effect,the prescription screening,physical properties investigation and bacteriostatic activity test were conducted.The oil phase,emulsifier and coemulsifier were optimized by determining the solubility of florfenicol in oil phases,emulsifiers and coemulsifier,respectively.The optimum formula was designed based on Kv of emulsifier-coemulsifier (Smix) and Smix-oil phase volume ratio which was selected using pseudo-ternary phase diagrm.Florfenicol nanoemulsion was distinguished by staining method,and the configuration of florfenicol nanoemulsion were measured by TEM,the particle size and Zeta potential were measured by laser particle size analyzer.The stability was investigated by a centrifugation and a long-term test.The antibacterial effect of florfenicol nanoemulsion on Staphylococcus aureus,Escherichia coli,Microbacterium and Bacillus subtilis was studied by a bacteriostatic activity test.The results showed that olive oil was the optimized oil phase,EL-40 was the optimized emulsifier,glycerin was the optimized coemulsifier,the optimized ratio of Kv was 2.0,and the volume ratio of Smix-oil was 8:2.The optimum formula of florfenicol nanoemulsion was the composition of fluorobenicol (120 mg),N,N-dimethylformamide(0.1 mL),olive oil (2 mL),EL-40(5.7 mL),propanol(2.3 mL),and distilled water(6 mL).The uniform size and no adhesion droplet shape of florfenicol nanoemulsion was O/W,with the average diameter of 28 nm and Zeta potential of -0.454 mV,maintained to be stable up to six months.The florfenicol nanoemulsion performanced a better antibacterial effect than the same concentration of fluorphenicol solution for four bacterias.The results demonstrated that the preparation method of florfenicol nanoemulsion was simple,feasible,and showed high stability and good bacteriostasis.The study proved that the florfenicol nanoemulsion had potential application value in the animal husbandry and aquaculture.

Key words: florfenicol; nano emulsion; pseudoternary phase diagrm; antibacterial effects

CLC Number:

S859.79

SHI Feifei, QIN Feng, WANG Xiujun, MA Qilun, GU Shutong, WEI Yalan, LI Yang. Preparation of Florfenicol Nanoemulsion and Observation of Its Antibacterial Effect[J]. , 2018, 45(10): 2936-2942.

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Preparation of Florfenicol Nanoemulsion and Observation of Its Antibacterial Effect

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