猪源大肠杆菌O157:H7体外氟苯尼考耐药诱导及对抗菌药物敏感性变化研究

doi:10.16431/j.cnki.1671-7236.2015.12.030

Abstract

Abstract: To preliminary investigate the mechanism of drug resistance and drug-resistant elimination of swine E.coli O157:H7,sub-inhibitory concentration in vitro drug-induced method was used in this study,for inducing two strains of E.coli O157:H7 becoming florfenicol highly resistant strains,and eliminated florfenicol resistance of florfenicol-resistant strains by continuous cultured without florfenicol pressure.The drug sensitivity and resistant genes of florfenicol-induced strains and florfenicol-resistance eliminated strains were detected.The results showed that compared with original strains,after drug-resistant induction by florfenicol,swine E.coli O157:H7 were resistant to florfenicol,amoxicillin,cefazolin,cephradine and cephalothin from high sensitivity,and resistant to cefotaxime,ofloxacin,ciprofloxacin and azithromycin from moderate sensitivity.However,after florfenicol-resistance eliminated,swine E.coli O157:H7 restored sensitivity to these drugs.In addition,florfenicol resistance gene,quinolone resistance genes and β-lactamase genes were found in the plasmids of swine E.coli O157:H7,the resistance genes were consistent with the resistance phenotype.According to the results,with the long-term presence of florfenicol pressure,swine E.coli O157:H7 resistant to florfenicol,and acquired penicillins,cephalosporins and quinolones cross-resistance;While swine E.coli O157:H7 was eliminated partial drug-resistance by continuous culture without florfenicol pressure.

Key words: swine E.coli O157:H7; florfenicol; drug-resistant induction; drug-resistant elimination; cross-resistance

CLC Number:

S852.61

FENG Shi-wen, LI Jun, ZENG Yun, YANG Wei, CHEN Ze-xiang, PAN Yan, PENG Hao. Study on Florfenicol-resistant Induction in vitro and Changes of Drug Sensitivity of Swine E.coli O157:H7[J]. , 2015, 42(12): 3315-3322.

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Study on Florfenicol-resistant Induction in vitro and Changes of Drug Sensitivity of Swine E.coli O157:H7

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