不同绵羊群体间心脏型脂肪酸结合蛋白基因第2外显子单核苷酸多态性的研究

Abstract

Abstract: PCR-SSCP approach was applied to test the single nucleotide polymorphisms (SNPs) at the exon 2 of heart fatty acid-binding protein (H-FABP) gene and four sheep populations that comprised of Northeast fine wool sheep, Small-Tailed Han sheep, Dorper sheep and Dorsets sheep were screened in this study. The results showed that four SNPs at 939(A/G), 980(G/A), 1013(A/C), 1018(T/C) and three genotypes were detected. During the Northeast fine wool sheep, Small-Tailed Han sheep and Dorper populations, the BB was the predominant genotype. While in Dorsets sheep, the AB was the predominant genotype. A chi-square analysis suggested that the allele frequencies and genotype frequencies of H-FABP gene were in Hardy-Weinberg equilibrium. Statistical analysis revealed an intermediate polymorphism information content (PIC) in the Northeast fine wool sheep, Dorper sheep and Dorsets populations (0.25＜PIC＜0.50), but a low PIC in the Small-Tailed Han sheep population (PIC＜0.25). Further analysis showed that the different groups had different degree of genetic variation.

Key words: sheep; heart fatty acid binding protein (H-FABP); SNPs

CLC Number:

Q753

ZHAO Xue, CAO Yang, JIN Hai-guo, ZHANG Li-chun, YU Yong-sheng, LUO Xiao-tong, LI De-yun. Studies on Single Nucleotide Polymorphisms at the Exon 2 of H-FABP Gene among Different Sheep Breeds[J]. , 2013, 40(4): 32-35.

References

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Studies on Single Nucleotide Polymorphisms at the Exon 2 of H-FABP Gene among Different Sheep Breeds

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