›› 2013, Vol. 40 ›› Issue (4): 167-173.

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Polymorphism of CETP Gene and Association Analysis with Production Performance-related in Dahe Blank Pig

LI Fu-quan1,2, YANG Wen2, GUO Bin3, HUO Jin-long4, SHI Xian-wei1   

  1. 1. Center of Biodiversity and Biotechnology, College of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201,China;
    2. Department of Biotechnology, Neijiang Vocational & Tecnical College, Neijiang, 641100, China;
    3. College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China;
    4. Key Laboratory of Bamma Mini-Pig Inbred Line of Yunan Province, Kunming 650201,China
  • Received:2012-10-17 Online:2013-04-20 Published:2013-04-19

Abstract: The associations between SNPs in the cholesteryl ester transfer protein (CETP) gene and production performance of pigs were reported in this study, the polymorphism of CETP gene was detected by a AvaⅡ RFLP in 334 Dahe Black pigs across F4 to F6 generations. These results showed that b alleles was excellent gene with frequencies of 0.5821, 0.6042 and 0.6885 in F4, F5 and F6 generations, respectively. Seven SNPs (19, 69, 132, 175, 203, 224 and 234 bp, respectively) were found by the homozygous samples sequencing. Associations between the founded AvaⅡ RFLP and birth weight, body weight at 45 days, body weight at 4 months, body weight at 6 months, body height at 6 months, body length at 6 months and cannon circumference at 6 months and back-fat thickness at 6 months were tested by least square analysis. The results showed that in F4 generation, body length at 6 months with Bb and bb genotype was higher than that with BB genotype (P＜0.05);cannon circumference at 6 months with bb genotype was higher than that with BB genotype (P＜0.05), that with Bb genotype was not significant (P＞0.05). In F5 generation, the birth weight with Bb and bb genotype was higher than that with BB genotype(P＜0.05), no significant difference between them (P＞0.05).In F6 generation, the birth weightand body weight at 45 dayswith bb genotype was higher than that with BB genotype (P＜0.05), that with Bb genotype was not significant (P＞0.05). In addition,the birth weight showed the higher significant difference between bb genetype and female/male (P＜0.05). These results suggested that the CETP gene had some effects on the growth traits of porcine,and the selection of b allect was favored with regard to the growth traits of porcine, this SNP might be considered as a potential genetic marker for improving growth.

Key words: Dahe Blank pigs; CETP; PCR-RFLP; production performance

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