牦牛病毒性腹泻病毒全基因测序及生物信息学分析

Abstract

Abstract: In this study, the bovine viral diarrhea virus (BVDV) strain Yak was used as material to sequence the complete genome.Nineteen pairs of primers were designed according to the complete genome sequence of BVDV-Ⅰ virus, the whole genome of BVDV strain Yak was cloned and sequenced. Sequence analysis showed that the genome was 12214 nucleotides (nt) in length, which contained a 288 nt 5'-untranslated region (UTR) and a 232 nt 3'-UTR. The complete genomic sequence of BVDV strain Yak had already submitted into GenBank (GenBank accession NO: JQ799141). Homologous analysis showed that homology of the whole genome sequence was low between BVDV strain Yak whole genome sequence and other 8 strains of BVDV whole genome sequences. Evolutionary tree results showed that the genetic evolution relationship was farther between BVDV strain Yak and other 8 strains of BVDV.

Key words: bovine viral diarrhea virus of yak; whole genome sequence analysis; bioinformatics analysis

CLC Number:

WANG Yan, LIU Ya-gang, WANG Rong, SUN Kai. Genome Sequencing and Bioinformatics Analysis of Bovine Viral Diarrhea Virus Strain Yak [J]. , 2013, 40(3): 33-37.

References

1 于吉峰.牦牛病毒性腹泻病毒5'-UTR、E0基因的克隆与序列分析及E0基因的原核表达与抗原性检测.成都:西南民族大学,2009.
2 王丹娜,吴明福,王君伟.牛病毒性腹泻疫苗的研究进展[J].畜牧与兽医,2006,38(7):51～53.
3 王文伯,刘亚刚,胡炳峰,等.牦牛病毒性腹泻病毒E1基因的克隆及生物信息学分析[J].中国畜牧兽医,2010,37(3):94～98.
4 王新平,刘红,宣华,等.绵羊感染牛病毒性腹泻病毒的调查研究[J].兽医大学学报,1993,13(3):219～221.
5 王新平,宣华,朱维正,等.鹿感染牛病毒性腹泻-黏膜病病毒的调查[J].中国畜禽传染病,1995,83(4):45～46.
6 西南民族学院"拉稀病"课题组.牦牛病毒性腹泻/黏膜病的研究[J].西南民族学院学报(畜牧兽医版),1984,40:1～7.
7 殷震,刘景华.动物病毒学[M].北京:科学出版社,1985.
8 Collett M S,Larson R,Belzer S K,et al.Proteins encoded by bovine viral diarrhea virus:The genomic organization of a pestivirus[J].Virology,1988,165(1):200～208.
9 Collett M,Larson R,Gold C,et al.Molecular cloning and nucleotide sequence of the pestivirus bovine viral diarrhea virus [J].Virus Res,2004,99(2):191～199.
10 Heuer C,Healy A,Zerbinbi C.Economic effects of exposure to bovine viral diarrhea virus on dairy herds in New Zealand[J].J Dairy Sci,2007,90:5418～5438.
11 Kalaycioglu A T.Bovine viral diarrhea virus(BVDV) diversity and vaccination.A review[J].Vet Q,2007,29(2):60～67.
12 Lindberg A,Brownlie J,Gunn G J,et al.The control of bovine viral diarrhea virus in Europe:Today and in the future[J].Rev Sci Tech,2006,25(3):961～979.
13 Massimo G,Ry H,Laura W,et al.Genoepidemiological evaluation of bovine viral diarrhea virus 2 species based on secondary structures in the 5'untranslated region[J].J Vet Med Sci,2008,70(6):571～580.
14 Moenning V,Frey H R,Lieber E,et al.Reproduction of mucosal disease with cytopathogenic bovine viral diarrhea virus selected in vitro[J].Vet Rec,1990,127:200～203.
15 Wengler G,Bradley D W,Collett M S,et al.Flaviviridea[M].Murphy F A,Fauquent C W,Bishiop D H L,et al.Virus taxonomy:Sixth report of the international committee on taxonomy of viruses.New York:Springer Verlag,1995.
16 Wensvoort G,Terpstra C.Bovine viral diarrhea virus infections in piglets born to sows vaccinated against swine fever with a contaminated vaccines [J].Res Vet Sci,1988,45:143～148.

[1]	LIU Jialong, ZHANG Yiwen, ZHANG Yueran, SUN Qijuan, CHEN Jian, HE Lei, JIA Yanyan, DING Ke, YU Zuhua. Cloning,Prokaryotic Expression and Bioinformatics Analysis of ELAVL1 Gene in Chicken [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1807-1817.
[2]	ZHUO Na, ZHU Zhongwu, LI Jing, HU Shixiang, WANG Jianchang, LIN Hua, CHEN Chaolin, HAN Diangang, AI Jun, CHEN Peifu. Bioinformatics Analysis and Eukaryotic Expression of ORF2 Protein of Swine Hepatitis E Virus [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1959-1970.
[3]	FAN Bingfeng, LI Wen, LIU Lixiang, ZHAO Xiangyuan, SHAO Jing, LIN Lefeng, SUN Huimin, ZHANG Xulin, XU Baozeng. Cloning and Bioinformatics Analysis of AANAT Gene in Mink [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1307-1318.
[4]	JIA Xinyue, MA Jing, ZHANG Yanyan, MA Xun, WANG Zhengrong, BO Xinwen. Bioinformatics Analysis and Prokaryotic Expression of EGR-07243 and EGR-07243 Genes of Echinococcus granulosus [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 847-858.
[5]	WEI Yirong, ZHENG Zihua, ZHANG Sanbao, SONG Ying, JIANG Haiyu, SUN Wenyue, CHENG Pengfei, LIU Yufan, ZOU Jianwei, HUANG Yanna, PAN Yan, JIANG Qinyang. Cloning,Bioinformatics Analysis and Tissue Expression of UCP1 Gene in Nubian Goats [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 440-450.
[6]	TANG Jingyu, DU Hanyu, MENG Chunchun, LIU Guangqing. Cloning,Bioinformatics Analysis and Subcellular Localization of Interferon-stimulated Gene 15 in Goats [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 2843-2854.
[7]	FU Yongzhi, MA Xiaojun, WANG Maohan, LI Zhe, CHEN Zhiyuan, QUAN Wei, ZHAO Han, SU Yongmei, WU Jianhua, ZHU Wenjin. Bioinformatics Analysis and Tissue Expression of MSTN Gene in Yangyuan Donkeys [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 2910-2919.
[8]	ZOU Jianwei, ZOU Juhong, SHEN Yujian, WEI Yi, ZHANG Sanbao, LIAN Zitong, XU Jianjian, SONG Ying, HUANG Yanna, WEI Yingming, JIANG Qinyang, ZHENG Zihua. Bioinformatics Analysis, Construction of Eukaryotic Expression Vector and Expression of LMCD1 Gene in Nubian Goats [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2033-2042.
[9]	ZHANG Limeng, LIU Aiju, LI Runting, LI Yuhua, LI Lin, NIE Xiaoning, WANG Linqing, CHEN Longxin. Cloning,Bioinformatics and Tissue Expression Analysis of CREBRF Gene in Ovis aries [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1599-1609.
[10]	LEI Xing, LI Tianfeng, LI Jiahao, MAN Xin, BAI Suying. Bioinformatics Analysis and Expression of Elovl1 Gene in Muskrat Scent Gland [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1201-1212.
[11]	SHEN Xiang, CHEN Linghui, YAO Jingjie, WANG Sanhu, BAI Yueyu, ZHANG Xiaojian. Cloning,Bioinformatics and Expression Analysis of Bovine CCR7 Gene [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1244-1252.
[12]	ZHANG Mingliang, GENG Yachun, LIAN Kaiqi, MA Lei, CHANG Ying, WANG Shuangshan, ZHANG Fuliang. Cloning,Bioinformatics Analysis and Truncated Fragment Expression for Truncated M Gene of Porcine Epidemic Diarrhea Virus [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1479-1487.
[13]	SHI Yuzhu, LUO Rutang, CHEN Chao, WU Xu, LI Ang. Cloning, Bioinformatics and Tissue Expression Analysis of GDF9 Genes in Muscovy Duck [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 817-829.
[14]	HOU Jinxing, WANG Zhanhang, ZHU Junru, JIANG Yue, LIU Shujuan, AN Xiaopeng. Cloning, Bioinformatics and Tissue Expression Profile Analysis of CSN1S1 Gene in Guanzhong Dairy Goats [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 857-865.
[15]	LI Yulong, LYU Shijie, ZHAI Yaying, ZHANG Zhijie, ZHU Xiaoting, ZHANG Zijing, CHEN Fuying, SHI Qiaoting, WANG Eryao. Cloning and Bioinformatics Analysis of POLB Gene in Jiaxian Red Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 887-896.

Genome Sequencing and Bioinformatics Analysis of Bovine Viral Diarrhea Virus Strain Yak 

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments