hsa-miR-21-5p靶基因预测及生物信息学分析

doi:10.16431/j.cnki.1671-7236.2023.11.004

Abstract

Abstract: 【Objective】 This study was aimed to analyze the sequence conservation and target genes of hsa-miR-21-5p by bioinformatics, so as to provide ideas for further exploring its function and regulatory mechanism during oocyte maturation.【Method】 The gene expression profiles (GSE31681) of cumulus cell at different stages of human oocytes during maturation were obtained from GEO database.The differentially expressed genes (DEGs) were screened by R software.The miRNAs targeted with DEGs were predicted by FunRich software and found the DEGs targeting by hsa-miR-21-5p to obtain miRNA-mRNA relationship pairs.The similarity and phylogenetic tree were analyzed and constructed by BLAST program and Mega 11.0 software, respectively, and the secondary structure of pre-miR-21-5p were predicted by ViennaRNA Web Services.The target genes of hsa-miR-21-5p were predicted using miRTarBase website, and GO function and KEGG pathway enrichment analysis was carried out.The STRING online database was accessed to develop the protein interaction network and the result was visualized through Cytoscape software.【Result】 A total of 7 DEGs targeted by hsa-miR-21-5p in cumulus cells corresponding to GV/MⅡ stages during oocyte maturation were screened.The sequence analysis of miR-21-5p among species showed that thesimilarity of human pre-miR-21-5p with Mus musculus, Rattus norvegicus, Bos taurus, Ovis aries, Tupaia chinensis and Macaca mulatta was over 95%, and miR-21-5p was highly conserved during evolution.The ViennaRNA Web Services prediction results showed that the secondary structure of pre-miR-21-5p had a typical single stem-loop structure.Target genes prediction revealed that hsa-miR-21-5p had 723 potential target genes.GO function analysis results showed that the target genes were mainly enriched in regulation of cell proliferation, intrinsic apoptotic signaling pathway, positive regulation of protein kinase activity and so on.KEGG pathway analysis results showed that the target genes were mainly enriched in MAPK and p53 signaling pathways.Protein interaction analysis results revealed that the proteins encoded by the target genes MYC, FOXO3 and STAT3 had more targeted relationships with other proteins, and involved in PI3K/Akt, JAK-STAT and other signaling pathways.【Conclusion】 miR-21-5p was highly conserved in biological evolution and played a role in oocyte maturation mainly by regulating the expression of related genes to regulate signaling pathways such as MAPK and p53 in cumulus cells.

Key words: miR-21-5p; target gene; cumulus cells; bioinformatics analysis

CLC Number:

Q78
S814.8

ZHANG Xinpei, WU Jiale, WU Fei, DI Shengwei. Target Gene Prediction and Bioinformatics Analysis of hsa-miR-21-5p[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(11): 4349-4358.

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Target Gene Prediction and Bioinformatics Analysis of hsa-miR-21-5p

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