小尾寒羊溶菌酶基因的克隆与差异表达分析

Abstract

Abstract: The male Small Tail Han sheep were inoculated with Brucella suis S2 vaccine strain, the suppression subtractive hybridization (SSH) library was constructed from buffy coat of sheep. After the recombinant plasmids were sequenced, the cDNA sequence of the conserved domain of lysozyme (LYZ) was recognized based on BLAST in GenBank, with the size of 770 bp, and continuously encoding 148 amino acid residuals, the encoded protein was a natural anti-infective substances.The sequence had been submitted to GenBank(JX263305).Quantitative Real-time PCR result showed that the LYZ gene was slightly up-regulated in buffy coat at 14 and 30 days post-challenge (dpc) without statistical significance (P>0.05), and then recovered to the normal level at 40 dpc.The lysozyme was further proved as an essential natural immune factor which laid the foundation for the effective prevention and control of brucellosis.

Key words: buffy coat; Brucella suis; lysozyme; differential expression analysis; Real-time PCR

CLC Number:

LI Chuang, XU Yun-ming, TANG Feng, ZOU De-ying, LIU Nan-nan, GUO Xing, YANG Yong-jie, ZHOU Yu, LIU Zeng-shan, LU Shi-ying, LU Cheng, REN Hong-lin. Cloning and Differential Expression Analysis of Lysozyme Gene in Small Tail Han Sheep[J]. , 2013, 40(3): 19-22.

References

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Cloning and Differential Expression Analysis of Lysozyme Gene in Small Tail Han Sheep

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