土槿皮乙酸B通过活性氧和有丝分裂原蛋白激酶诱导MCF-7细胞凋亡的研究

Abstract

Abstract: The assay was aimed to study the relationship of ROS and MAPK family protein in pseudolaric acid B (PAB)-induced MCF-7 cell apoptosis. Contrast phase microscope was used to observe cellular morphologic alternation; MTT analysis detected the inhibitory ratio; flow cytometric analysis of DCFH-DA staining determined the level of ROS, and Western blotting detected the protein expression. We found that PAB induced MCF-7 cell apoptosis through increasing ROS and increased the phosphoration of JNK and decrease the phosphoration of ERK.

Key words: pseudolaric acid B; ROS; apoptosis; MAPK; MCF-7 family protein

CLC Number:

LIU Chun-yu, ZHENG Gui-bin, YU Jing-hua. Study of Pseudolaric Acid B Induced Apoptosis through ROS Regulating MAPK in MCF-7 Cells[J]. China Animal Husbandry & Veterinary Medicine, 2013, 40(3): 146-148.

References

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Study of Pseudolaric Acid B Induced Apoptosis through ROS Regulating MAPK in MCF-7 Cells

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