Abstract: In order to study the anti-porcine parvovirus (PPV) activity, propolis was diluted to ten concentrations with maintenance medium and added into PK-15 foster system to determine the maximal safe concentration of propolis by MTT(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) method. And then propolis was diluted to five concentrations with maintenance medium from their safe concentration and added into PK-15 monolayers by three adding drug patterns (pre-adding drug pattern, post-adding drug pattern and simultaneous adding pattern) to observe the antiviral activity of propolis against PPV on PK-15 by MTT assay. The concentration of PPV in PK-15 foster system was determined by fluorescence quantitative PCR assay to observe the effect of eliminating PPV of propolis on PK-15. The results showed that propolis could promote the anti-PPV ability of PK-15 and reduce the content of PPV in foster system of PK-15 in concentration of 31.2 to 250 μg/mL, and the effects had related to concentrations and adding drug patterns, the higher concentration,the better effectiveness,and the anti-PPV activity of propolis in pre-adding drug pattern was the best in three adding drug patterns.

Key words: propolis; porcine parvovirus; antivirus