以减毒鼠伤寒沙门氏菌为载体的重组NDV-HN口服疫苗的构建与鉴定

Abstract

Abstract: The study was aimed to construct prokaryotic expression vector of NDV HN gene using attenuated Salmonella typhimurium SL1344ΔcrpΔasd as a carrier. The HN gene was cloned from the plasmid pMD18-T-HN by polymerase chain reaction (PCR) and inserted into the vector pYA3493. Electricity transformation method was used in this recombinant plasmid pYA3493-HN transformating to recepted state Salmonella typhimurium SL1344ΔcrpΔasd. The recombinant plasmid pYA3493-HN was identified by digestion of endonuclease, PCR and sequencing. The results confirmed that recombinant attenuated Salmonella typhimurium vaccine SL1344ΔcrpΔasd(pYA3493-HN) was constructed successfully, which provided a foundation for research and development of NDV HN orally genetic engineering live vaccine.

Key words: Newcastle disease; HN gene; prokaryotic expression vector pYA3493; attenuated Salmonella typhimurium

CLC Number:

S852.612

TIAN Fang-hua, CHENG Xiang-chao, ZHANG Chun-jie, LI Yin-ju, LI Jing, LIU Yi-chen. Construction and Identification of Recombinant Attenuated Salmonella typhimurium Oral NDV-HN Vaccine[J]. , 2013, 40(11): 59-63.

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Construction and Identification of Recombinant Attenuated Salmonella typhimurium Oral NDV-HN Vaccine

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