猪繁殖与呼吸综合征病毒感染猪肺脏组织环腺苷酸磷酸二酯酶的活性及其mRNA表达变化

Abstract

Abstract: The objective of this paper was to study the activity and expression change of cAMP-PDE in lung of porcine infected with porcine reproductive and respiratory syndrome virus (PRRSV) through the examination of PDE activity and gene expression,and provided data for the application of PDE inhibitors basis.Acquisition lung of porcine infected with PRRSV,cAMP-PDE activity was calculated by cyclic nucleotides content change examined with HPLC before and after the PDE reaction,and cAMP-PDE mRNA expression was detected by Real-time PCR. The results showed that the activity of cAMP-PDE extracted from lung of PRRS group was significantly higher than that of the control group (P<0.05), and in addition to PDE4A, mRNA expression of PDE4B, PDE4C, PDE4D, PDE7A, PDE7B, PDE8A and PDE8B were higher than that of the control group.Those results indicated that there were some abnormal changes of cAMP-PDE in the inflammation progress of lungs infected by PRRSV,and suggested that specific inhibitor of the cAMP-PDE might ease the inflammation injury in lung of porcine infected with PRRSV.

Key words: porcine reproductive and respiratory syndrome (PRRS); lung; cyclic nucleotides; phosphodiesterase

CLC Number:

S852.65

ZHANG Qian, LI Jia, SUN Sheng-yong, JIANG Dai-xun, CHEN Wu. Activity and mRNA Expression of Cyclic Nucleotide-specific Phosphodiesterase in the Lung of Porcine Infected with Porcine Reproductive and Respiratory Syndrome Virus[J]. , 2013, 40(10): 6-10.

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Activity and mRNA Expression of Cyclic Nucleotide-specific Phosphodiesterase in the Lung of Porcine Infected with Porcine Reproductive and Respiratory Syndrome Virus

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