China Animal Husbandry and Veterinary Medicine ›› 2020, Vol. 47 ›› Issue (5): 1342-1351.doi: 10.16431/j.cnki.1671-7236.2020.05.007

• Physiology and Biochemistry • Previous Articles     Next Articles

Establishment of Ascites Syndrome Model in Broiler Induced by Multi-factor and Its Effect on Diastolic-systolic Factors of Vascular Endothelium in Lung Tissue

GUO Yurong1, CUI Liang1, SU Xiaofei2, WANG Zhuo1, ZHU Yubo3, LI Peiqi1, ZHANG Jian1, SUN Hanyan1, YUN Shiyu4, DUAN Zhibian1   

  1. 1. College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, China;
    2. Shanxi Animal Husbandry and Veterinary School, Taiyuan 030024, China;
    3. College of Animal Science, Henan Institute of Science and Technology, Xinxiang 453003, China;
    4. Institute of Industrial Crops, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, China
  • Online:2020-05-20 Published:2020-05-18

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Abstract: This experiment was aimed to study the experimental model of broiler ascites syndrome (AS) by multi-factor method and its effect on diastolic-systolic factors of vascular endothelium in lung tissue.A total of 158 healthy Ross broilers were randomly divided into normal group,low temperature model group and multi-factor model group.At 25,35 and 45 days of age,the clinical symptoms,post-mortem change and the pathological change of lung tissue were observed,the body weight,the incidence of AS and ascites heart index (AHI) were measured,and the change of NO,ET-1 and NOS were detected,and the expression of ETAR and VEGF genes in lung tissue were detected by Real-time quantitative PCR.The results showed that the AHI of broilers at each day-age in the low temperature and the multi-factor model groups was extremely significantly higher than that in the normal group (P<0.01),the AHI of broilers at each day-age in the multi-factor model group was significantly higher than that in the low temperature model group (P<0.05).The body weight of broilers at 35 and 45 days of age in the low temperature model group and each day-age in the multi-factor model group were significantly or extremely significantly lower than that in the normal group (P<0.05,P<0.01).The incidence of AS at 35 and 45 days of age in the low temperature model group and each day-age in the multi-factor model group were extremely significantly higher than that in the normal group (P<0.01).At 25 days of age,the incidence of AS in the multi-factor model group was extremely significantly higher than that in the low temperature model group (P<0.01).At 35 days of age,lung tissue of broilers with AS structure was damaged,endothelial cells were shed,and smooth muscle arrangement was disturbed.Compared with the normal group,the contents of NO and ET-1,iNOS activity,ETAR and VEGF mRNA expression in lung tissue at 35 and 45 days of age in the low temperature and the multi-factor model groups were significantly or extremely significantly increased (P<0.05,P<0.01),the ratio of NO to ET-1 and the activity of cNOS and TNOS were significantly or extremely significantly reduced (P<0.05,P<0.01).Experimental ascites syndrome model in broiler could be successfully established induced by multi-factor method with low temperature,high sodiu and high energy feed.The multi-factor model method could reflect the diversity of disease factors better than the low temperature model method.The expression disorder of diastolic-systolic factors of vascular endothelium in lung tissue was involved in the occurrence of broiler AS.

Key words: multi-factor method; ascites syndrome (AS); lung tissue; diastolic-systolic factors of vascular endothelium

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