Abstract: This study was to aim to use ELISA method for rapid screening of the aflatoxins B₁(AFB₁) residues. Coupled AFB₁ to bull serum albumin(BSA) and ovalbumin(OVA) to obtain AFB₁-BSA and AFB₁-OVA respectively. Specific antibody against AFB₁ was prepared with AFB₁-BSA and ELISA kit,and had been successfully developed for detecting AFB₁ residues. The IC₅₀ of the kit was 128.8 ng/L,linear detection range was 50~1800 ng/L with the lowest detection limit less than 100 ng/kg. The recovery of AFB₁ in edible oil,peanut and cereal was more than 71.3%. Coefficient of variation for repeatability tests was less than 14.2%.Therefore,the kit was simple,fast,sensitive,and would be play an important role in AFB₁ residues detection in the plant food.

Key words: aflatoxins B₁; enzyme-linked immunosorbent assay; kit