进出口动物中新霉素ELISA检测方法的建立

doi:10.16431/j.cnki.1671-7236.2016.12.012

Abstract

Abstract:

This experiment used carbodiimide get egg albumin synthesis of artificial antigens coupling neomycin,through square test to determine the best antigen packaged concentration and antibody dilution ratio,mass concentration logarithm of neomycin as abscissa,inhibition rate of antibody as the ordinate drawing standard curve,established ELISA fast detection techniques of neomycin in edible animal for import and export.Methods of recovery and specific test had been done in further research.Results showed that the test dilution ratio of optimum synthetic antigen was 1:200,the best dilution multiple of antibodies was 1:2 000, half inhibitory concentration(IC₅₀) was 6.99 ng/mL;The minimum detection limit of the method was 40 ng/mL,and cross reaction rate of gentamicin, kanamycin, streptomycin,tobramycin,amikacin and dihydrostreptomycin were less than 0.1%,the average of recovery rate was 83.77%.The range of standarding curve in 1.5~40 ng/mL was linear,the correlation coefficient was 0.987,the equation of standarding curve was y=-37.66x+94.592;Compared with HPLC method,the operation was simple and fast.The test results showed that the enzyme-linked immunosorbent method of detecting neomycin was rapid,efficient and specific.

Key words: import and export food animals; neomycin; enzyme-linked immunosorbent assay (ELISA); detection method

CLC Number:

S859.2

SUN Ming-jun, QIU Fang, ZHANG Jin-ling, ZHENG Xiao-long. Establishment of ELISA Detection Method for Neomycin in Import and Export Animal[J]. , 2016, 43(12): 3163-3169.

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Establishment of ELISA Detection Method for Neomycin in Import and Export Animal

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