牛α-乳清蛋白基因5'调控区多态性研究

Abstract

Abstract: In order to analyze the polymorphisms of LALBA gene 5'regulation region in cattle. Two cattle breeds (Wuchuan Black cattle and Guizhou Holstein cow) with significant difference in breeds property were selected to construct DNA pools, a total of 1126 bp sequences of LALBA 5'regulation region and part of exon 1 were obtained by designing 1 pair specific primers. The results showed that 5 single nucleotide polymorphisms (SNPs) were found in the 5'regulation region which included T-114C, C-166T, A-225C, C-344T, T-778C respectively and T-114C was specific to Wuchuan Black cattle. Furthermore, bioinformatics tools were used to predict the core region of the promoter and identify various transcription factors binding sites. It demonstrated that 5 new transcription factors binding sites emerged while 11 previous transcription factors binding sites disappeared which included one located in the core region of promoter results from the SNPs found in this study, the remarkable change of secondary structure of RNA was also detected but the CpG islands was not found by using various softwares.

Key words: Guizhou Holstein cow; Wuchuan Black cattle; LALBA gene; SNP; promoter

CLC Number:

YANG Yong-qiang, GONG Yu, JIAO Ren-gang, HUI Yan-ting, LIU Ruo-yu. Study on the Polymorphisms of LALBA Gene 5' Regulation Region in Cattle[J]. , 2013, 40(1): 136-140.

References

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Study on the Polymorphisms of LALBA Gene 5' Regulation Region in Cattle

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