山羊腺苷单磷酸脱氨酶1基因启动子区单核苷酸多态性研究

Abstract

Abstract: The assay was aimed to screen the polymorphisms of AMPD1 gene promoter and analyze the effect of SNPs on function elements of promoter. Three goat breeds (Small-xiang goat, Qianbei ma goat, Nubian goat) were selected to construct DNA pools, SNPs sites were screened by direct sequencing subsequently and analyzed by BLAST. The results showed that 5 single nucleotide polymorphisms (SNPs) were found in the promoter region which included T-614A, G-326A, G-309A, T-287C and T-165C. Furthermore, bioinformatics tools were used to predict the core region of the promoter and identify various transcription factors binding sites. It demonstrated that 7 new transcription factors binding sites emerged while 9 previous transcription factors binding sites disappeared based on the SNPs found in this study, the remarkable change of secondary structure of RNA were also detected but the range of CpG islands were not predicted by the analysis of various softwares.

Key words: AMPD1; SNP; promoter; Small-xiang goat; Qianbei ma goat; Nubian goat

CLC Number:

S816.79

WANG De-feng, YANG Yong-qiang, HAN Yong. Study on the SNPs of AMPD1 Gene Promoter Region in Goat[J]. , 2013, (7): 127-132.

References

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Study on the SNPs of AMPD1 Gene Promoter Region in Goat

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