两种纯化兔抗酸性红73免疫球蛋白G方法的比较研究

Abstract

Abstract: To compare the effects of saturated ammonium sulfate method and caprylic acid-ammonium sulfate method for purifying the rabbit anti-AR73 IgG. The IgG were tested in protein content,relative molecular weight, the antibody titer by BCA method,sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and indirect enzyme linked immunosorbent assay (ELISA).The results indicated that the protein content of IgG by saturated ammonium sulfate method was 36.44 mg/mL and the antibody titer was 1.6×10⁵, the protein content of IgG by caprylic acid-ammonium sulfate method was 15.79 mg/mL, and the antibody titer was 3.2×10⁵. The purity of purified antibody by caprylic acid-ammonium sulfate method was higher than that by saturated ammonium sulfate method.

Key words: acid red 73; acid red GR; saturated ammonium sulfate; caprylic acid-ammonium sulfate

CLC Number:

S829.1

CHANG Xiang-cai, YANG Xiao-nong, ZHU Zi-feng, CHEN Rui, LIU Ling-ying. Comparison of Two Methods for Purifying the Rabbit Antibody of Acid Red 73 IgG[J]. China Animal Husbandry & Veterinary Medicine, 2013, (7): 124-126.

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Comparison of Two Methods for Purifying the Rabbit Antibody of Acid Red 73 IgG

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