两种纯化兔抗酸性红73免疫球蛋白G方法的比较研究

Abstract

Abstract: To compare the effects of saturated ammonium sulfate method and caprylic acid-ammonium sulfate method for purifying the rabbit anti-AR73 IgG. The IgG were tested in protein content,relative molecular weight, the antibody titer by BCA method,sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and indirect enzyme linked immunosorbent assay (ELISA).The results indicated that the protein content of IgG by saturated ammonium sulfate method was 36.44 mg/mL and the antibody titer was 1.6×10⁵, the protein content of IgG by caprylic acid-ammonium sulfate method was 15.79 mg/mL, and the antibody titer was 3.2×10⁵. The purity of purified antibody by caprylic acid-ammonium sulfate method was higher than that by saturated ammonium sulfate method.

Key words: acid red 73; acid red GR; saturated ammonium sulfate; caprylic acid-ammonium sulfate

CLC Number:

S829.1

CHANG Xiang-cai, YANG Xiao-nong, ZHU Zi-feng, CHEN Rui, LIU Ling-ying. Comparison of Two Methods for Purifying the Rabbit Antibody of Acid Red 73 IgG[J]. , 2013, (7): 124-126.

[1]	ZHONG Hualing, HE Congcong, WU Bangyuan. Effects of Fresh Alfalfa and Full-price Pellet Feed on the Antioxidant Properties and Muscle Structure of Rabbit Stomach [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 143-150.
[2]	BO Xinyu, TAO Xiaozhao, REN Zhanjun, DONG Xianggui, WANG Shuhui. Research Progress on β-NGF in Induced Ovulation in Rabbits [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(11): 4288-4295.
[3]	WANG Yuanlang, DING Haisheng, ZHAO Huiling, HUANG Dongwei. Research Progress on Genetic Breeding and Reproduction of Domestic and International Rabbits in 2021 [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(10): 3918-3924.
[4]	TIE Yanan, YANG Xixi, WANG Zhiliang, WANG Xinrong. Morphological and Histological Characteristics of Testis and Epididymis of Hyla Rabbits at Different Ages Before Sexual Maturity [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2645-2653.
[5]	WANG Zhiliang, YANG Xixi, TIE Yanan, FU Xiaoyu, WANG Xinrong. Effects of Superovulation on Morphology and Hormone Levels of Reproductive Organs in Rabbits [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2250-2258.
[6]	WANG Yuanlang, DING Haisheng, ZHAO Huiling, HUANG Dongwei. Research Progress on Genetic Breeding and Reproduction of Domestic and International Rabbits in 2020 [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(1): 248-255.
[7]	JIANG Yixuan, WANG Yaling, XING Shanshan, SONG Guohua, XU Huifen, LI Ming. Cloning, Bioinformatics Analysis and Expression Profile Construction of MSTN Gene in New Zealand White Rabbits [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(10): 3501-3511.
[8]	SHANG Shufeng, WANG Qi, GUO Sufen, CHEN Zhiyuan, WANG Yangke. Effects of the Dark Plum Powder Extraction on Contraction of Small Intestine Smooth Muscle of Rabbits in vitro and Its Mechanism [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(2): 477-483.
[9]	ZHANG Liping, WANG Jianfeng, LI Zhenzhen, LEI Qiong, DANG Haoqian, LIU Qinghua. Effects of Different Sources of Fiber Diet on Apparent Digestibility,Duodenal Tissue Morphology and Cecal Fiber Digestive Enzyme Activity in Fujian Yellow Rabbits [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(2): 501-508.
[10]	DING Haisheng, HUANG Dongwei, WANG Yong, CHENG Guanglong, MA Rongxing, YANG Yongxin, ZHAO Huiling. Research Progress on Genetic Breeding and Reproduction of Domestic and International Rabbits in 2019 [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(12): 3976-3984.
[11]	CHEN Mutian, REN Yan, ZHANG Lei, LI Jing, XIE Sijie, ZHANG Yan, ZHANG Ming, MEI Xiuli. Effect of CsA on the Expression of IL-1β and IL-6 in Endometrial Stomas Cells Induced by LPS [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(10): 3351-3360.
[12]	ZHENG Jiangping, LIU Ning, BAI Xuerui, ZHANG Feike. Effect of Tetramethylpyrazine on Listeria monocytogenes Infection and Innate Immunity of Rex Rabbits [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(5): 1531-1538.
[13]	ZHENG Jiangping, LIU Ning, BAI Xuerui, ZHANG Feike. Effect of Yeast Polysaccharide on Growth Performance,Bacterial Load and Endogenous Antimicrobial Peptides in Rabbits Infected with Campylobacter jejuni Expression [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(4): 1099-1108.
[14]	ZHANG Zhaoxia, YANG Lin, DING Haisheng, HUANG Dongwei, WANG Yong, CHENG Guanglong, ZHAO Huiling. Research Progress on Genetic Breeding and Reproduction of Domestic and International Rabbit in 2018 [J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(11): 3307-3315.
[15]	YU Chunying, YANG Yanyan, ZHOU Jianhua, LIN Wei, WANG Xunli. Study on Genetic Diversity of Microsatellite Markers in Three Rabbit Breeds [J]. , 2018, 45(12): 3486-3496.

Comparison of Two Methods for Purifying the Rabbit Antibody of Acid Red 73 IgG

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments