MDCC-MSB1细胞酵母双杂交cDNA表达文库的构建与鉴定

Abstract

Abstract: To construct cDNA expressing library using MDCC-MSB1 cells in a good state, cDNA were synthesized using biotin-conjugated Oligo (dT) primer in the 5'end. The doublestrand cDNA was ligated to adapter and passed the cDNA size fractionation columns. The cDNA entry library was constructed by BP recombination. The library had a high titer of 1?10⁵ CFU/mL, and contained a total clones of 1.1?10⁶ CFU with an average inserts size was about 1190 bp. The constructed cDNA expression library by LR recombination had a titer of 1?10⁵ CFU/mL and contained total clones of 1.2?10⁶ CFU, with an average inserts size of 1087 bp. The cDNA expression libraries would be a valuable resource for identification of cell receptors of CIAV on the MDCC-MSB1 cells and its cellular tropism.

Key words: MDCC-MSB1 cell lines; cDNA library; chicken infectious anemia; yeast two-hybrid

CLC Number:

Q786

CUI Peng-peng, GAO Hong-lei, LI Kai, GAO Li, GAO Yu-long, QI Xiao-le, WANG Xiao-mei. Construction and Characterization of Yeast Two-hybrid cDNA Expression Library of MDCC-MSB1[J]. , 2013, (5): 22-25.

References

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Construction and Characterization of Yeast Two-hybrid cDNA Expression Library of MDCC-MSB1

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