Abstract: One pair of specific primers was designed based on inserting sequence IS1111 of Q fever Coxiella burnetii, Real-time turbidity assay of loop-mediated isothermal amplification (LAMP) was developed for indentification Q fever. The recombinant plasmid containing the target sequence was constructed to detect the sensitivity of LAMP with the isothermal temperature. The method could detect 10 of the plasmid copy number. The established Real-time turbidity assay of LAMP method showed no cross reaction with M.tuberculosis, C.psittaci, Brucella.spp and DNA of bovine bloods were negative. Real-time turbidity assay of LAMP was developed in this study and had high sensitivity and specificity,it had a good application prospects in the detection and identification of the Q fever.

Key words: Q feve; Coxiella burnetii; loop-mediated isothermal amplification (LAMP); Real-time turbidity assay