猪钙蛋白酶抑制蛋白基因外显子9多态性检测及其对山西白猪背膘厚的影响

Abstract

Abstract: A total of 416 pigs from Duroc, Large White, Landrace, Shanxi White, Shanxi Black and Mashen were used to detect the polymorphisms of calpastatin (CAST) gene exon 9 by PCR- SSCP. Five SNPs were detected in the amplified region, which were T37G, A150G, C167T, C193T, and C256T, of them, the mutations of C193T and C256T located in the CAST gene exon 9 were missense mutation, which gave rise to the changes from threonine to isoleucine and from threonine to methionine, respectively. Seven haplotypes of A, B, C, D, E, F and G were detected in the examined populations. The distributions of the haplotypes in different pig breeds were anastomosed with the pig economic types. There were three haplotypes of A, B and C in the imported pigs of Large White and Duroc, and the frequency of haplotype B was greater than those of A and C, which were 0.40 in Large White and 0.53 in Duroc. Only two haplotypes of B and C were detected in Landrace, the frequency of haplotype C was greater than that of haplotype of B. Except of the haplotypes of A, B, and C, a new haplotype of E was detected in Mashen pig population, the frequency was 0.16. The haplotypes had no significant effect on the backfat thickness measured alive in Shanxi White pigs.

Key words: pig; PCR-SSCP; calpastatin gene; backfat thickness

CLC Number:

S813.3

ZHAO Xian-ping, LI Wei, WANG Yan-ling, HE Xiao-li, GAO Peng-fei, Guo Xiao-hong, LI Bu-gao, CAO Guo-qing. Study on the Polymorphisms of CAST Gene Exon 9 and its Effect on Backfat Thickness in Shanxi White Pig[J]. , 2013, (5): 161-165.

References

1 邓正德,连林生.大河乌猪钙蛋白酶抑制蛋白基因、脂肪细胞定向和分化因子1基因多态性及其与肉质性状的关联分析[J].养猪,2012(3):57~60.
2 左北瑶,钱宏光.CAST基因研究进展[J].中国草食动物,2011,31(6):64~68.
3 刘香,虞德兵,邱新深,等.苏太猪CAST基因多态性与肉质性状的相关性研究[J].畜牧与兽医, 2010, 42(4):15~18.
4 孙立彬,孟和,李婧,等.猪钙蛋白酶抑制蛋白基因PCR-RFLP多态性与肉质性状及背膘厚间的关系分析[J].上海交通大学学报(农业科学版),2005,23(1):57~62.
5 孙立彬,孟和,李婧,等.梅山猪等5个群体中钙蛋白酶抑制蛋白基因的多态性及其与肉质和背膘厚的关系[J].农业生物技术学报,2006,14 (2): 156~161.
6 吴旧生,徐宁迎.金皮猪肉质相关组织学特征与CAST基因HinfⅠ多态性的关系研究[J]. 分子细胞生物学报,2007, 40(5):295~300.
7 张立春,李赵志,金鑫,等.延边黄牛CAST基因16内含子基因多态性与肉质性状相关性分析[J].中国畜牧杂志, 2011, 47(15): 12~15.
8 张菊,杜立新,魏彩虹,等.绵羊CAST基因2型和4型转录本的克隆及特性分析[J].遗传,2009,31(11):1107~1112.
9 张璐,曹洪战,芦春莲,等.4个猪种CAST基因与背膘厚关系的研究[J].黑龙江畜牧兽医,2009(1):8~9.
10 邱莫寒,鲜凌瑾,张平.钙蛋白酶系统基因的研究进展[J].安徽农业科学,2009,37(28):13628~13631.
11 曹果清,薛尚君,杨文平,等.山西白猪高产仔母系繁殖性能测定[J].中国畜牧兽医,2009,36(4):111~114.
12 彭英林,柳小春,施启顺,等.钙蛋白酶抑制蛋白(CAST)基因型与营养水平互作对猪胴体性状的影响[J].农业生物技术学报,2009,17(5):767~772.
13 Ciobanu D C, Bastiaansen J W M, Lonergan S M, et al. New alleles in calpastatin gene are associated with meat quality traits in pigs[J]. J Anim Sci, 2004, 82(10): 2829~2839.
14 Ernst C W, Robic A, Yerle M, et al. Mapping of caplastatin and three microsatellites to porcine chromosome 2q2.1-2.4[J]. Anim Genet, 1998, 29(3):212~215.
15 Jolanta K, Wojciech K, Mariusz P, et al. Preliminary observations on the effect of calpastatin gene (CAST) polymorphism on carcass traits in pigs[J]. Animal Science Papers and Reports, 2003, 21(2):87~95.
16 Kocwin-Podsiada M, Kury J, Krzcio E, et al. The interaction between calpastatin and RYR1 genes for some pork quality traits[J].Meat Science, 2003, 65(2):731~735.
17 Krzcio E, Kocwin-Podsiada M, Kuryl J, et al. the effect of interaction between genotype CAST/RsaⅠ (calpastatin) and MYOG/MspⅠ (myogenin) on carcass and meat quality in pigs free of RYR1T allele[J]. Meat Science, 2008,80(4):1106~1115.
18 Xue H L, Xu L X. Capastatin genotype is associated with meat quality traits in pigs[J]. Chinese Journal of Biochemistry and Molecular Biology, 2009, 25(7):615~619.

[1]	WEI Mingbang, BIANBA Qiongda, XIAO Qingqing, DUAN Mengqi, CHAMBA Yangzom, SHANG Peng. Cloning,Bioinformatics and Expression Analysis of CDKN1B Gene in Tibetan Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2196-2206.
[2]	ZHANG Fangwei, ZHANG Qi, LEI Liangliang, SUN Wusheng, ZHANG Di, ZHANG Yunpeng, ZHANG Jingbo, WANG Xiuquan, ZHANG Jing, ZHANG Shumin. Polymorphism of HBEGF Gene and Its Association with Reproductive Traits in Songliao Black Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2370-2379.
[3]	PAN Pengcheng, LEI Zongquan, LI Xian, HU Xiangyun, QIN Qiantao, QIN Zhaoxian, GUAN Zhihui, CHEN Baojian, XIE Bingkun. Bioinformatics Analysis,Eukaryotic Expression Vector Construction and Tissue Expression of MYL2 Gene in Luchuan Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1796-1806.
[4]	OUYANG Kun, WEI Limin, SUN Ruiping, XIE Yali, QI Qi, LI Xiang, LIU Quanwei, CHAO Zhe, ZHANG Yongliang, CHEN Ting. Effects of Curcumin on Growth Performance,Feed Apparent Digestibility and Serum Biochemical Indexes in Hainan Tunchang Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1867-1875.
[5]	WU Jingxuan, SUN Zheng, MA Xuelian, ZHANG Liyang, LIAO Xiudong, LUO Xugang, LI Sufen, LYU Lin. A Survey on Magnesium Contents in Feed Resources for Livestock and Poultry in China [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1888-1898.
[6]	WANG Zeping, WANG Feifei, ZHANG Ruiming, FU Yanfeng, ZHAO Weimin, DAI Chaohui, CHENG Jinhua, LIAO Chao, LI Hui. Analysis on Polymorphism of SLA-1 Gene and Its Disease Resistance Potential in Suzi Pig [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1918-1927.
[7]	LIU Hongrun, ZHU Siran, FENG Lingli, ZHANG Kun, YAN Gang, WANG Yubin, ZHANG Shuai, JIANG Shan, XU Di, LAN Ganqiu, LIANG Jing. Cloning,Bioinformatics Analysis and Tissue Expression Localization of CDO1 Gene in Large White Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1352-1363.
[8]	YAO Kang, CAO Shuting, JIANG Zongyong, WANG Li. The Research Progress of Lysine Application and Requirement in Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1364-1374.
[9]	ZHANG Rui, MU Chunyu, TANG Qingping, BU Zhu, YANG Mingjun, XIA Aiping, YANG Xiaoming. Polymorphism of GnIH Gene and Its Correlation with Egg-laying Traits in Pigeons [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1412-1421.
[10]	ZHANG Fangwei, ZHANG Qi, ZHANG Yunpeng, LI Xin, ZHANG Jingbo, ZHANG Shumin, YU Yongsheng. Polymorphism of FOXA2 Gene Exon 3 and Its Association with Reproductive Traits in Songliao Black Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1444-1451.
[11]	TAN Yuancheng, LUO Hualun, ZHANG Yiyu, YANG Suan, WANG Yingtong, WANG Chunyuan. Polymorphism of MyoG Gene and Its Association with Body Size and Meat Traits in White Feather King Pigeons [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1461-1471.
[12]	HAN Dayong, ZHOU Chunbao, NI Ligang, CHEN Zhangyan, ZHAO Jinming. Genetic Diversity of mtDNA D-loop Sequence in Sujiang Pig [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1472-1479.
[13]	YANG Zhi, YU Jiaxin, YANG Linfu, WANG Kang, LI Guanzong, DUAN Gang, YANG Fumei, LIU Zhiqi, ZHOU Ke, HE Jun, DAI Feiyan. Study on the Therapeutic Effect of Modified Sini Decoction on Piglet Diarrhea [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1707-1717.
[14]	CHANG Yitong, ZHANG Wei, PENG Yinglin, CHEN Chen. Evolutionary Analysis,Target Gene Prediction and Tissue Expression Analysis of miR-192 [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 882-892.
[15]	LIU Zhen, ZHOU Xueqin, XU Fei, LI Zhefeng, SUN Hanxue, LI Guoqin, TAO Zhengrong, GUAN Mei, ZHENG Qiang, DIAO Xinping, LU Lizhi. Effects of Coated Sodium Butyrate on Growing Performance,Slaughter Performance, Immune Organ Index and Digestive Enzyme Activity of Breeding Pigeons and Squabs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 998-1006.

Study on the Polymorphisms of CAST Gene Exon 9 and its Effect on Backfat Thickness in Shanxi White Pig

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments