猪滋养层干细胞样细胞的分离培养

Abstract

Abstract: Trophoblast stem cells (TSc) were the precursors of the differentiated cells of the placenta. It was associated with embryo implantation. Isolating trophoblast stem cells in vitro provided research foundation for trophoblastic development. TSc had been successfully derivated from preimplantation embryo, there were also many reasearches about porcine trophoblast stem cells(pTSc), but no reports indicated derivating 6 d pTSc. After broken the zona pellucida,we fixed the blastocyst on feeder under sterile condition. The porcine trophoblatic stem-like cell was intially analysed by morphogenesis and RT-PCR. We found the porcine trophoblastic stem-like cell combined tightly and present epithelioid-like cell. The clone was smooth,the ratio of the nucleus and cytoplasm was mass. The porcine trophoblastic-like cell was confirmed expressing Cdx2 gene by RT-PCR. We successfully derived the porcine trophoblastic stem-like cell from 6 d pathenogonidium blastcyst. The result provided some foundation for follow-up studies of porcine trophoblast development.

Key words: porcine; throphoblast stem cell; in vitro blastocyst; isolation and culture

CLC Number:

S813.1⁺1

SONG Zhi-qiang, LIU Chang, FENG Chong, LONG Chuan, ZHAO Qin-li, LIU Zhong-hua, PAN Deng-ke. Isolation and Cultivation of Porcine Trophoblastic Stem-like Cell Lines[J]. , 2013, (5): 156-160.

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Isolation and Cultivation of Porcine Trophoblastic Stem-like Cell Lines

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